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@ARTICLE{Oleksiuk:127242,
      author       = {O. Oleksiuk and M. Abba and K. C. Tezcan and W. Schaufler
                      and F. Bestvater and N. Patil and U. Birk and M. Hafner and
                      P. Altevogt$^*$ and C. Cremer and H. Allgayer$^*$},
      title        = {{S}ingle-{M}olecule {L}ocalization {M}icroscopy allows for
                      the analysis of cancer metastasis-specific mi{RNA}
                      distribution on the nanoscale.},
      journal      = {OncoTarget},
      volume       = {6},
      number       = {42},
      issn         = {1949-2553},
      address      = {[S.l.]},
      publisher    = {Impact Journals LLC},
      reportid     = {DKFZ-2017-03267},
      pages        = {44745 - 44757},
      year         = {2015},
      abstract     = {We describe a novel approach for the detection of small
                      non-coding RNAs in single cells by Single-Molecule
                      Localization Microscopy (SMLM). We used a modified
                      SMLM-setup and applied this instrument in a first
                      proof-of-principle concept to human cancer cell lines. Our
                      method is able to visualize single microRNA (miR)-molecules
                      in fixed cells with a localization accuracy of 10-15 nm, and
                      is able to quantify and analyse clustering and localization
                      in particular subcellular sites, including exosomes. We
                      compared the metastasis-site derived (SW620) and primary
                      site derived (SW480) human colorectal cancer (CRC) cell
                      lines, and (as a proof of principle) evaluated the
                      metastasis relevant miR-31 as a first example. We observed
                      that the subcellular distribution of miR-31 molecules in
                      both cell lines was very heterogeneous with the largest
                      subpopulation of optically acquired weakly metastatic cells
                      characterized by a low number of miR-31 molecules, as
                      opposed to a significantly higher number in the majority of
                      the highly metastatic cells. Furthermore, the highly
                      metastatic cells had significantly more miR-31-molecules in
                      the extracellular space, which were visualized to
                      co-localize with exosomes in significantly higher numbers.
                      From this study, we conclude that miRs are not only
                      aberrantly expressed and regulated, but also differentially
                      compartmentalized in cells with different metastatic
                      potential. Taken together, this novel approach, by providing
                      single molecule images of miRNAs in cellulo can be used as a
                      powerful supplementary tool in the analysis of miRNA
                      function and behaviour and has far reaching potential in
                      defining metastasis-critical subpopulations within a given
                      heterogeneous cancer cell population.},
      keywords     = {MIRN31 microRNA, human (NLM Chemicals) / MicroRNAs (NLM
                      Chemicals)},
      cin          = {G300 / G360},
      ddc          = {610},
      cid          = {I:(DE-He78)G300-20160331 / I:(DE-He78)G360-20160331},
      pnm          = {317 - Translational cancer research (POF3-317)},
      pid          = {G:(DE-HGF)POF3-317},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:26561203},
      pmc          = {pmc:PMC4792589},
      doi          = {10.18632/oncotarget.6297},
      url          = {https://inrepo02.dkfz.de/record/127242},
}