%0 Journal Article
%A Rabionet, Mariona
%A Bayerle, Aline
%A Jennemann, Richard
%A Heid, Hans
%A Fuchser, Jens
%A Marsching, Christian
%A Porubsky, Stefan
%A Bolenz, Christian
%A Guillou, Florian
%A Gröne, Hermann-Josef
%A Gorgas, Karin
%A Sandhoff, Roger
%T Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors.
%J Human molecular genetics
%V 24
%N 17
%@ 1460-2083
%C Oxford
%I Oxford Univ. Press
%M DKFZ-2017-03360
%P 4792 - 4808
%D 2015
%X Somatic cell cytokinesis was shown to involve the insertion of sphingolipids (SLs) to midbodies prior to abscission. Spermatogenic midbodies transform into stable intercellular bridges (ICBs) connecting clonal daughter cells in a syncytium. This process requires specialized SL structures. (1) Using high resolution-mass spectrometric imaging, we show in situ a biphasic pattern of SL synthesis with testis-specific anchors. This pattern correlates with and depends on ceramide synthase 3 (CerS3) localization in both, pachytene spermatocytes until completion of meiosis and elongating spermatids. (2) Blocking the pathways to germ cell-specific ceramides (CerS3-KO) and further to glycosphingolipids (glucosylceramide synthase-KO) in mice highlights the need for special SLs for spermatid ICB stability. In contrast to somatic mitosis these SLs require ultra-long polyunsaturated anchors with unique physico-chemical properties, which can only be provided by CerS3. Loss of these anchors causes enhanced apoptosis during meiosis, formation of multinuclear giant cells and spermatogenic arrest. Hence, testis-specific SLs, which we also link to CerS3 in human testis, are quintessential for male fertility.
%K Fatty Acids (NLM Chemicals)
%K RNA, Messenger (NLM Chemicals)
%K Sphingolipids (NLM Chemicals)
%K LASS3 protein, mouse (NLM Chemicals)
%K Sphingosine N-Acyltransferase (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:26045466
%R 10.1093/hmg/ddv204
%U https://inrepo02.dkfz.de/record/127335