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001     127335
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024 7 _ |a 10.1093/hmg/ddv204
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024 7 _ |a pmid:26045466
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024 7 _ |a 0964-6906
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024 7 _ |a 1460-2083
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037 _ _ |a DKFZ-2017-03360
041 _ _ |a eng
082 _ _ |a 570
100 1 _ |a Rabionet, Mariona
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245 _ _ |a Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors.
260 _ _ |a Oxford
|c 2015
|b Oxford Univ. Press
336 7 _ |a article
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336 7 _ |a ARTICLE
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336 7 _ |a Journal Article
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520 _ _ |a Somatic cell cytokinesis was shown to involve the insertion of sphingolipids (SLs) to midbodies prior to abscission. Spermatogenic midbodies transform into stable intercellular bridges (ICBs) connecting clonal daughter cells in a syncytium. This process requires specialized SL structures. (1) Using high resolution-mass spectrometric imaging, we show in situ a biphasic pattern of SL synthesis with testis-specific anchors. This pattern correlates with and depends on ceramide synthase 3 (CerS3) localization in both, pachytene spermatocytes until completion of meiosis and elongating spermatids. (2) Blocking the pathways to germ cell-specific ceramides (CerS3-KO) and further to glycosphingolipids (glucosylceramide synthase-KO) in mice highlights the need for special SLs for spermatid ICB stability. In contrast to somatic mitosis these SLs require ultra-long polyunsaturated anchors with unique physico-chemical properties, which can only be provided by CerS3. Loss of these anchors causes enhanced apoptosis during meiosis, formation of multinuclear giant cells and spermatogenic arrest. Hence, testis-specific SLs, which we also link to CerS3 in human testis, are quintessential for male fertility.
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650 _ 7 |a Fatty Acids
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650 _ 7 |a RNA, Messenger
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650 _ 7 |a Sphingolipids
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650 _ 7 |a LASS3 protein, mouse
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650 _ 7 |a Sphingosine N-Acyltransferase
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700 1 _ |a Bayerle, Aline
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700 1 _ |a Jennemann, Richard
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700 1 _ |a Heid, Hans
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700 1 _ |a Fuchser, Jens
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700 1 _ |a Marsching, Christian
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700 1 _ |a Porubsky, Stefan
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700 1 _ |a Bolenz, Christian
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700 1 _ |a Guillou, Florian
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700 1 _ |a Gröne, Hermann-Josef
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700 1 _ |a Gorgas, Karin
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700 1 _ |a Sandhoff, Roger
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773 _ _ |a 10.1093/hmg/ddv204
|g Vol. 24, no. 17, p. 4792 - 4808
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