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@ARTICLE{Ramin:127349,
      author       = {M. Ramin$^*$ and N. Denk$^*$ and J. Schenkel$^*$},
      title        = {{T}he role of diet and housing-temperature in the
                      production of genetically modified mouse embryos and their
                      developmental capacity after cryopreservation.},
      journal      = {Theriogenology},
      volume       = {84},
      number       = {8},
      issn         = {0093-691X},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DKFZ-2017-03374},
      pages        = {1306 - 1313},
      year         = {2015},
      abstract     = {Mutant mouse lines are unique models with an enormous
                      scientific potential. Cryopreservation of preimplantation
                      embryos or of spermatozoa is a common approach to save those
                      lines. The breeding of a line can be discontinued if
                      sufficient specimens have been cryopreserved. Prerequisites
                      to economically cryopreserve embryos are high yields of
                      embryos prepared from donors and a high recovery rate after
                      revitalization. Diets for laboratory animals are often
                      produced from phytoestrogen-containing soy; the present
                      study shows that feeding the donor animals with a
                      phytoestrogen-poor diet is more efficient compared to a
                      phytoestrogen-containing, soy-based diet. Additionally, a
                      uterotrophic bioassay indicating the estrogenic role of
                      compounds showed a significant increase of the relative
                      uterus size of females fed with a phytoestrogen-rich diet.
                      The role of the housing-temperature was investigated, too,
                      showing that a housing-temperature of 24 °C results in the
                      best embryo yields. The production of two-cell embryos is
                      more economic than the production of eight-cell embryos.
                      Investigating the recovery rate of frozen/thawed embryos, a
                      very high recovery rate was determined when both, two- and
                      eight-cell embryos were thawed. However, the capacity to
                      develop to the next embryonic stage in vitro was
                      dramatically reduced when two-cell embryos were compared to
                      eight-cell embryos. After embryo transfer, the sex ratio
                      became uneven and more males were delivered. This effect
                      might be due to the procedures to which animals and embryos
                      were subjected. These data show that many parameters can
                      influence the production of animals when using
                      (frozen/thawed) embryos. These parameters need continuous
                      surveillance.},
      keywords     = {Phytoestrogens (NLM Chemicals)},
      cin          = {W430 / W410},
      ddc          = {590},
      cid          = {I:(DE-He78)W430-20160331 / I:(DE-He78)W410-20160331},
      pnm          = {311 - Signalling pathways, cell and tumor biology
                      (POF3-311)},
      pid          = {G:(DE-HGF)POF3-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:26259536},
      doi          = {10.1016/j.theriogenology.2015.07.010},
      url          = {https://inrepo02.dkfz.de/record/127349},
}