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000127681 1001_ $$aVaillant, Catherine$$b0
000127681 245__ $$aSerpine2/PN-1 Is Required for Proliferative Expansion of Pre-Neoplastic Lesions and Malignant Progression to Medulloblastoma.
000127681 260__ $$aLawrence, Kan.$$bPLoS$$c2015
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000127681 520__ $$aMedulloblastomas are malignant childhood brain tumors that arise due to the aberrant activity of developmental pathways during postnatal cerebellar development and in adult humans. Transcriptome analysis has identified four major medulloblastoma subgroups. One of them, the Sonic hedgehog (SHH) subgroup, is caused by aberrant Hedgehog signal transduction due to mutations in the Patched1 (PTCH1) receptor or downstream effectors. Mice carrying a Patched-1 null allele (Ptch1∆/+) are a good model to study the alterations underlying medulloblastoma development as a consequence of aberrant Hedgehog pathway activity.Transcriptome analysis of human medulloblastomas shows that SERPINE2, also called Protease Nexin-1 (PN-1) is overexpressed in most medulloblastomas, in particular in the SHH and WNT subgroups. As siRNA-mediated lowering of SERPINE2/PN-1 in human medulloblastoma DAOY cells reduces cell proliferation, we analyzed its potential involvement in medulloblastoma development using the Ptch1∆/+ mouse model. In Ptch1∆/+ mice, medulloblastomas arise as a consequence of aberrant Hedgehog pathway activity. Genetic reduction of Serpine2/Pn-1 interferes with medulloblastoma development in Ptch1∆/+ mice, as ~60% of the pre-neoplastic lesions (PNLs) fail to develop into medulloblastomas and remain as small cerebellar nodules. In particular the transcription factor Atoh1, whose expression is essential for development of SHH subgroup medulloblastomas is lost. Comparative molecular analysis reveals the distinct nature of the PNLs in young Ptch1∆/+Pn-1Δ/+ mice. The remaining wild-type Ptch1 allele escapes transcriptional silencing in most cases and the aberrant Hedgehog pathway activity is normalized. Furthermore, cell proliferation and the expression of the cell-cycle regulators Mycn and Cdk6 are significantly reduced in PNLs of Ptch1∆/+Pn-1Δ/+ mice.Our analysis provides genetic evidence that aberrant Serpine2/Pn-1 is required for proliferation of human and mouse medulloblastoma cells. In summary, our analysis shows that Serpine2/PN-1 boosts malignant progression of PNLs to medulloblastomas, in which the Hedgehog pathway is activated in a SHH ligand-independent manner.
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000127681 650_7 $$2NLM Chemicals$$aHedgehog Proteins
000127681 650_7 $$2NLM Chemicals$$aPTCH protein, human
000127681 650_7 $$2NLM Chemicals$$aPatched Receptors
000127681 650_7 $$2NLM Chemicals$$aPatched-1 Receptor
000127681 650_7 $$2NLM Chemicals$$aPtch1 protein, mouse
000127681 650_7 $$2NLM Chemicals$$aReceptors, Cell Surface
000127681 650_7 $$2NLM Chemicals$$aSerpin E2
000127681 650_7 $$0EC 3.4.24.35$$2NLM Chemicals$$aMatrix Metalloproteinase 9
000127681 7001_ $$aValdivieso, Paola$$b1
000127681 7001_ $$aNuciforo, Sandro$$b2
000127681 7001_ $$0P:(DE-He78)4c28e2aade5f44d8eca9dd8e97638ec8$$aKool, Marcel$$b3$$udkfz
000127681 7001_ $$aSchwarzentruber-Schauerte, Alexandra$$b4
000127681 7001_ $$aMéreau, Hélène$$b5
000127681 7001_ $$aCabuy, Erik$$b6
000127681 7001_ $$aLobrinus, Johannes A$$b7
000127681 7001_ $$0P:(DE-He78)f746aa965c4e1af518b016de3aaff5d9$$aPfister, Stefan$$b8$$udkfz
000127681 7001_ $$aZuniga, Aimée$$b9
000127681 7001_ $$aFrank, Stephan$$b10
000127681 7001_ $$aZeller, Rolf$$b11
000127681 773__ $$0PERI:(DE-600)2267670-3$$a10.1371/journal.pone.0124870$$gVol. 10, no. 4, p. e0124870 -$$n4$$pe0124870 -$$tPLoS one$$v10$$x1932-6203$$y2015
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