000127703 001__ 127703
000127703 005__ 20240228140944.0
000127703 0247_ $$2doi$$a10.1016/j.tiv.2015.03.005
000127703 0247_ $$2pmid$$apmid:25795400
000127703 0247_ $$2ISSN$$a0887-2333
000127703 0247_ $$2ISSN$$a1879-3177
000127703 037__ $$aDKFZ-2017-03726
000127703 041__ $$aeng
000127703 082__ $$a610
000127703 1001_ $$aVichi, S.$$b0
000127703 245__ $$aCell type-specific expression and localization of cytochrome P450 isoforms in tridimensional aggregating rat brain cell cultures.
000127703 260__ $$aAmsterdam [u.a.]$$bElsevier Science$$c2015
000127703 3367_ $$2DRIVER$$aarticle
000127703 3367_ $$2DataCite$$aOutput Types/Journal article
000127703 3367_ $$0PUB:(DE-HGF)16$$2PUB:(DE-HGF)$$aJournal Article$$bjournal$$mjournal$$s1521722059_15724
000127703 3367_ $$2BibTeX$$aARTICLE
000127703 3367_ $$2ORCID$$aJOURNAL_ARTICLE
000127703 3367_ $$00$$2EndNote$$aJournal Article
000127703 520__ $$aWithin the Predict-IV FP7 project a strategy for measurement of in vitro biokinetics was developed, requiring the characterization of the cellular model used, especially regarding biotransformation, which frequently depends on cytochrome P450 (CYP) activity. The extrahepatic in situ CYP-mediated metabolism is especially relevant in target organ toxicity. In this study, the constitutive mRNA levels and protein localization of different CYP isoforms were investigated in 3D aggregating brain cell cultures. CYP1A1, CYP2B1/B2, CYP2D2/4, CYP2E1 and CYP3A were expressed; CYP1A1 and 2B1 represented almost 80% of the total mRNA content. Double-immunolabeling revealed their presence in astrocytes, in neurons, and to a minor extent in oligodendrocytes, confirming the cell-specific localization of CYPs in the brain. These results together with the recently reported formation of an amiodarone metabolite following repeated exposure suggest that this cell culture system possesses some metabolic potential, most likely contributing to its high performance in neurotoxicological studies and support the use of this model in studying brain neurotoxicity involving mechanisms of toxication/detoxication.
000127703 536__ $$0G:(DE-HGF)POF3-313$$a313 - Cancer risk factors and prevention (POF3-313)$$cPOF3-313$$fPOF III$$x0
000127703 588__ $$aDataset connected to CrossRef, PubMed,
000127703 650_7 $$2NLM Chemicals$$aIsoenzymes
000127703 650_7 $$09035-51-2$$2NLM Chemicals$$aCytochrome P-450 Enzyme System
000127703 7001_ $$aSandström von Tobel, J.$$b1
000127703 7001_ $$aGemma, S.$$b2
000127703 7001_ $$aStanzel, S.$$b3
000127703 7001_ $$0P:(DE-He78)bb6a7a70f976eb8df1769944bf913596$$aKopp-Schneider, A.$$b4$$udkfz
000127703 7001_ $$aMonnet-Tschudi, F.$$b5
000127703 7001_ $$aTestai, E.$$b6
000127703 7001_ $$aZurich, M. G.$$b7
000127703 773__ $$0PERI:(DE-600)1501079-x$$a10.1016/j.tiv.2015.03.005$$gVol. 30, no. 1 Pt A, p. 176 - 184$$n1 Pt A$$p176 - 184$$tToxicology in vitro$$v30$$x0887-2333$$y2015
000127703 909CO $$ooai:inrepo02.dkfz.de:127703$$pVDB
000127703 9101_ $$0I:(DE-588b)2036810-0$$6P:(DE-He78)bb6a7a70f976eb8df1769944bf913596$$aDeutsches Krebsforschungszentrum$$b4$$kDKFZ
000127703 9131_ $$0G:(DE-HGF)POF3-313$$1G:(DE-HGF)POF3-310$$2G:(DE-HGF)POF3-300$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bGesundheit$$lKrebsforschung$$vCancer risk factors and prevention$$x0
000127703 9141_ $$y2015
000127703 915__ $$0StatID:(DE-HGF)0420$$2StatID$$aNationallizenz
000127703 915__ $$0StatID:(DE-HGF)0300$$2StatID$$aDBCoverage$$bMedline
000127703 915__ $$0StatID:(DE-HGF)0310$$2StatID$$aDBCoverage$$bNCBI Molecular Biology Database
000127703 915__ $$0StatID:(DE-HGF)0100$$2StatID$$aJCR$$bTOXICOL IN VITRO : 2015
000127703 915__ $$0StatID:(DE-HGF)0200$$2StatID$$aDBCoverage$$bSCOPUS
000127703 915__ $$0StatID:(DE-HGF)0600$$2StatID$$aDBCoverage$$bEbsco Academic Search
000127703 915__ $$0StatID:(DE-HGF)0030$$2StatID$$aPeer Review$$bASC
000127703 915__ $$0StatID:(DE-HGF)0199$$2StatID$$aDBCoverage$$bThomson Reuters Master Journal List
000127703 915__ $$0StatID:(DE-HGF)0110$$2StatID$$aWoS$$bScience Citation Index
000127703 915__ $$0StatID:(DE-HGF)0150$$2StatID$$aDBCoverage$$bWeb of Science Core Collection
000127703 915__ $$0StatID:(DE-HGF)0111$$2StatID$$aWoS$$bScience Citation Index Expanded
000127703 915__ $$0StatID:(DE-HGF)1030$$2StatID$$aDBCoverage$$bCurrent Contents - Life Sciences
000127703 915__ $$0StatID:(DE-HGF)1050$$2StatID$$aDBCoverage$$bBIOSIS Previews
000127703 915__ $$0StatID:(DE-HGF)9900$$2StatID$$aIF < 5
000127703 9201_ $$0I:(DE-He78)C060-20160331$$kC060$$lBiostatistik$$x0
000127703 980__ $$ajournal
000127703 980__ $$aVDB
000127703 980__ $$aI:(DE-He78)C060-20160331
000127703 980__ $$aUNRESTRICTED