TY  - JOUR
AU  - Vichi, S.
AU  - Sandström von Tobel, J.
AU  - Gemma, S.
AU  - Stanzel, S.
AU  - Kopp-Schneider, A.
AU  - Monnet-Tschudi, F.
AU  - Testai, E.
AU  - Zurich, M. G.
TI  - Cell type-specific expression and localization of cytochrome P450 isoforms in tridimensional aggregating rat brain cell cultures.
JO  - Toxicology in vitro
VL  - 30
IS  - 1 Pt A
SN  - 0887-2333
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - DKFZ-2017-03726
SP  - 176 - 184
PY  - 2015
AB  - Within the Predict-IV FP7 project a strategy for measurement of in vitro biokinetics was developed, requiring the characterization of the cellular model used, especially regarding biotransformation, which frequently depends on cytochrome P450 (CYP) activity. The extrahepatic in situ CYP-mediated metabolism is especially relevant in target organ toxicity. In this study, the constitutive mRNA levels and protein localization of different CYP isoforms were investigated in 3D aggregating brain cell cultures. CYP1A1, CYP2B1/B2, CYP2D2/4, CYP2E1 and CYP3A were expressed; CYP1A1 and 2B1 represented almost 80
KW  - Isoenzymes (NLM Chemicals)
KW  - Cytochrome P-450 Enzyme System (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:25795400
DO  - DOI:10.1016/j.tiv.2015.03.005
UR  - https://inrepo02.dkfz.de/record/127703
ER  -