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@ARTICLE{Vichi:127703,
      author       = {S. Vichi and J. Sandström von Tobel and S. Gemma and S.
                      Stanzel and A. Kopp-Schneider$^*$ and F. Monnet-Tschudi and
                      E. Testai and M. G. Zurich},
      title        = {{C}ell type-specific expression and localization of
                      cytochrome {P}450 isoforms in tridimensional aggregating rat
                      brain cell cultures.},
      journal      = {Toxicology in vitro},
      volume       = {30},
      number       = {1 Pt A},
      issn         = {0887-2333},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DKFZ-2017-03726},
      pages        = {176 - 184},
      year         = {2015},
      abstract     = {Within the Predict-IV FP7 project a strategy for
                      measurement of in vitro biokinetics was developed, requiring
                      the characterization of the cellular model used, especially
                      regarding biotransformation, which frequently depends on
                      cytochrome P450 (CYP) activity. The extrahepatic in situ
                      CYP-mediated metabolism is especially relevant in target
                      organ toxicity. In this study, the constitutive mRNA levels
                      and protein localization of different CYP isoforms were
                      investigated in 3D aggregating brain cell cultures. CYP1A1,
                      CYP2B1/B2, CYP2D2/4, CYP2E1 and CYP3A were expressed; CYP1A1
                      and 2B1 represented almost $80\%$ of the total mRNA content.
                      Double-immunolabeling revealed their presence in astrocytes,
                      in neurons, and to a minor extent in oligodendrocytes,
                      confirming the cell-specific localization of CYPs in the
                      brain. These results together with the recently reported
                      formation of an amiodarone metabolite following repeated
                      exposure suggest that this cell culture system possesses
                      some metabolic potential, most likely contributing to its
                      high performance in neurotoxicological studies and support
                      the use of this model in studying brain neurotoxicity
                      involving mechanisms of toxication/detoxication.},
      keywords     = {Isoenzymes (NLM Chemicals) / Cytochrome P-450 Enzyme System
                      (NLM Chemicals)},
      cin          = {C060},
      ddc          = {610},
      cid          = {I:(DE-He78)C060-20160331},
      pnm          = {313 - Cancer risk factors and prevention (POF3-313)},
      pid          = {G:(DE-HGF)POF3-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:25795400},
      doi          = {10.1016/j.tiv.2015.03.005},
      url          = {https://inrepo02.dkfz.de/record/127703},
}