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000127723 0247_ $$2doi$$a10.1016/j.ab.2013.12.006
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000127723 0247_ $$2ISSN$$a0003-2697
000127723 0247_ $$2ISSN$$a1096-0309
000127723 037__ $$aDKFZ-2017-03746
000127723 041__ $$aeng
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000127723 1001_ $$0P:(DE-HGF)0$$aKübler, Dieter$$b0$$eFirst author
000127723 245__ $$aPhosphorylation of multifunctional galectins by protein kinases CK1, CK2, and PKA.
000127723 260__ $$aSan Diego, Calif.$$bElsevier$$c2014
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000127723 520__ $$aPhosphorylation is known to have a strong impact on protein functions. We analyzed members of the lectin family of multifunctional galectins as targets of the protein kinases CK1, CK2, and PKA. Galectins are potent growth regulators able to bind both glycan and peptide motifs at intra- and extracellular sites. Performing in vitro kinase assays, galectin phosphorylation was detected by phosphoprotein staining and autoradiography. The insertion of phosphoryl groups varied to a large extent depending on the type of kinase applied and the respective galectin substrate. Sites of phosphorylation observed in the recombinant galectins were determined by a strategic combination of phosphopeptide enrichment and nano-ultra-performance liquid chromatography tandem mass spectrometry (nanoUPLC-MS/MS). By in silico modeling, phosphorylation sites were visualized three-dimensionally. Our results reveal galectin-type-specific Ser-/Thr-dependent phosphorylation beyond the known example of galectin-3. These data are the basis for functional studies and also illustrate the analytical sensitivity of the applied methods for further work on human lectins.
000127723 536__ $$0G:(DE-HGF)POF3-311$$a311 - Signalling pathways, cell and tumor biology (POF3-311)$$cPOF3-311$$fPOF III$$x0
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000127723 650_7 $$2NLM Chemicals$$aGalectins
000127723 650_7 $$2NLM Chemicals$$aRecombinant Proteins
000127723 650_7 $$0EC 2.7.11.1$$2NLM Chemicals$$aCasein Kinase I
000127723 650_7 $$0EC 2.7.11.1$$2NLM Chemicals$$aCasein Kinase II
000127723 650_7 $$0EC 2.7.11.11$$2NLM Chemicals$$aCyclic AMP-Dependent Protein Kinases
000127723 7001_ $$aSeidler, Jörg$$b1
000127723 7001_ $$aAndré, Sabine$$b2
000127723 7001_ $$aKumar, Sonu$$b3
000127723 7001_ $$0P:(DE-He78)fd29481ff828e64dd1002b7a4efc5932$$aSchwartz-Albiez, Reinhard$$b4$$udkfz
000127723 7001_ $$0P:(DE-HGF)0$$aLehmann, Wolf-Dieter$$b5
000127723 7001_ $$aGabius, Hans-Joachim$$b6
000127723 773__ $$0PERI:(DE-600)1461105-3$$a10.1016/j.ab.2013.12.006$$gVol. 449, p. 109 - 117$$p109 - 117$$tAnalytical biochemistry$$v449$$x0003-2697$$y2014
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