TY  - JOUR
AU  - Kuhmann, Christine
AU  - Li, Carmen
AU  - Kloor, Matthias
AU  - Salou, Mariam
AU  - Weigel, Christoph
AU  - Schmidt, Christopher R
AU  - Ng, Linda W C
AU  - Tsui, Wendy W Y
AU  - Leung, Suet Y
AU  - Yuen, Siu T
AU  - Becker, Natalia
AU  - Weichenhan, Dieter
AU  - Plass, Christoph
AU  - Schmezer, Peter
AU  - Chan, Tsun L
AU  - Popanda, Odilia
TI  - Altered regulation of DNA ligase IV activity by aberrant promoter DNA methylation and gene amplification in colorectal cancer.
JO  - Human molecular genetics
VL  - 23
IS  - 8
SN  - 1460-2083
CY  - Oxford
PB  - Oxford Univ. Press
M1  - DKFZ-2017-03779
SP  - 2043 - 2054
PY  - 2014
AB  - Colorectal cancer (CRC) presents as a very heterogeneous disease which cannot sufficiently be characterized with the currently known genetic and epigenetic markers. To identify new markers for CRC we scrutinized the methylation status of 231 DNA repair-related genes by methyl-CpG immunoprecipitation followed by global methylation profiling on a CpG island microarray, as altered expression of these genes could drive genomic and chromosomal instability observed in these tumors. We show for the first time hypermethylation of MMP9, DNMT3A and LIG4 in CRC which was confirmed in two CRC patient groups with different ethnicity. DNA ligase IV (LIG4) showed strong differential promoter methylation (up to 60
KW  - Biomarkers, Tumor (NLM Chemicals)
KW  - LIG4 protein, human (NLM Chemicals)
KW  - RNA, Messenger (NLM Chemicals)
KW  - DNA (Cytosine-5-)-Methyltransferase (NLM Chemicals)
KW  - DNA methyltransferase 3A (NLM Chemicals)
KW  - MMP9 protein, human (NLM Chemicals)
KW  - Matrix Metalloproteinase 9 (NLM Chemicals)
KW  - DNA Ligases (NLM Chemicals)
KW  - DNA Ligase ATP (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:24282031
DO  - DOI:10.1093/hmg/ddt599
UR  - https://inrepo02.dkfz.de/record/127757
ER  -