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000127890 0247_ $$2ISSN$$a0370-2952
000127890 0247_ $$2ISSN$$a1477-9137
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000127890 037__ $$aDKFZ-2017-03912
000127890 041__ $$aeng
000127890 082__ $$a570
000127890 1001_ $$aZwerger, Monika$$b0
000127890 245__ $$aAltering lamina assembly reveals lamina-dependent and -independent functions for A-type lamins.
000127890 260__ $$aCambridge$$bCompany of Biologists Limited$$c2015
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000127890 520__ $$aLamins are intermediate filament proteins that form a fibrous meshwork, called the nuclear lamina, between the inner nuclear membrane and peripheral heterochromatin of metazoan cells. The assembly and incorporation of lamin A/C into the lamina, as well as their various functions, are still not well understood. Here, we employed designed ankyrin repeat proteins (DARPins) as new experimental tools for lamin research. We screened for DARPins that specifically bound to lamin A/C, and interfered with lamin assembly in vitro and with incorporation of lamin A/C into the native lamina in living cells. The selected DARPins inhibited lamin assembly and delocalized A-type lamins to the nucleoplasm without modifying lamin expression levels or the amino acid sequence. Using these lamin binders, we demonstrate the importance of proper integration of lamin A/C into the lamina for nuclear mechanical properties and nuclear envelope integrity. Finally, our study provides evidence for cell-type-specific differences in lamin functions.
000127890 536__ $$0G:(DE-HGF)POF3-312$$a312 - Functional and structural genomics (POF3-312)$$cPOF3-312$$fPOF III$$x0
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000127890 650_7 $$2NLM Chemicals$$aLamin Type A
000127890 650_7 $$2NLM Chemicals$$aLamin Type B
000127890 650_7 $$2NLM Chemicals$$aLamins
000127890 7001_ $$aRoschitzki-Voser, Heidi$$b1
000127890 7001_ $$aZbinden, Reto$$b2
000127890 7001_ $$aDenais, Celine$$b3
000127890 7001_ $$0P:(DE-He78)7892a89fee19b8e3912c7423d660765d$$aHerrmann, Harald$$b4$$udkfz
000127890 7001_ $$aLammerding, Jan$$b5
000127890 7001_ $$aGrütter, Markus G$$b6
000127890 7001_ $$aMedalia, Ohad$$b7
000127890 773__ $$0PERI:(DE-600)1483099-1$$a10.1242/jcs.171843$$gVol. 128, no. 19, p. 3607 - 3620$$n19$$p3607 - 3620$$tJournal of cell science$$v128$$x1477-9137$$y2015
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000127890 9141_ $$y2015
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