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@ARTICLE{Zwerger:127890,
      author       = {M. Zwerger and H. Roschitzki-Voser and R. Zbinden and C.
                      Denais and H. Herrmann$^*$ and J. Lammerding and M. G.
                      Grütter and O. Medalia},
      title        = {{A}ltering lamina assembly reveals lamina-dependent and
                      -independent functions for {A}-type lamins.},
      journal      = {Journal of cell science},
      volume       = {128},
      number       = {19},
      issn         = {1477-9137},
      address      = {Cambridge},
      publisher    = {Company of Biologists Limited},
      reportid     = {DKFZ-2017-03912},
      pages        = {3607 - 3620},
      year         = {2015},
      abstract     = {Lamins are intermediate filament proteins that form a
                      fibrous meshwork, called the nuclear lamina, between the
                      inner nuclear membrane and peripheral heterochromatin of
                      metazoan cells. The assembly and incorporation of lamin A/C
                      into the lamina, as well as their various functions, are
                      still not well understood. Here, we employed designed
                      ankyrin repeat proteins (DARPins) as new experimental tools
                      for lamin research. We screened for DARPins that
                      specifically bound to lamin A/C, and interfered with lamin
                      assembly in vitro and with incorporation of lamin A/C into
                      the native lamina in living cells. The selected DARPins
                      inhibited lamin assembly and delocalized A-type lamins to
                      the nucleoplasm without modifying lamin expression levels or
                      the amino acid sequence. Using these lamin binders, we
                      demonstrate the importance of proper integration of lamin
                      A/C into the lamina for nuclear mechanical properties and
                      nuclear envelope integrity. Finally, our study provides
                      evidence for cell-type-specific differences in lamin
                      functions.},
      keywords     = {Lamin Type A (NLM Chemicals) / Lamin Type B (NLM Chemicals)
                      / Lamins (NLM Chemicals)},
      cin          = {B065},
      ddc          = {570},
      cid          = {I:(DE-He78)B065-20160331},
      pnm          = {312 - Functional and structural genomics (POF3-312)},
      pid          = {G:(DE-HGF)POF3-312},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:26275827},
      pmc          = {pmc:PMC4610210},
      doi          = {10.1242/jcs.171843},
      url          = {https://inrepo02.dkfz.de/record/127890},
}