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| Home > Publications database > Altering lamina assembly reveals lamina-dependent and -independent functions for A-type lamins. > print |
| 001 | 127890 | ||
| 005 | 20240228140955.0 | ||
| 024 | 7 | _ | |a 10.1242/jcs.171843 |2 doi |
| 024 | 7 | _ | |a pmid:26275827 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC4610210 |2 pmc |
| 024 | 7 | _ | |a 0021-9533 |2 ISSN |
| 024 | 7 | _ | |a 0370-2952 |2 ISSN |
| 024 | 7 | _ | |a 1477-9137 |2 ISSN |
| 024 | 7 | _ | |a altmetric:6230448 |2 altmetric |
| 037 | _ | _ | |a DKFZ-2017-03912 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Zwerger, Monika |b 0 |
| 245 | _ | _ | |a Altering lamina assembly reveals lamina-dependent and -independent functions for A-type lamins. |
| 260 | _ | _ | |a Cambridge |c 2015 |b Company of Biologists Limited |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1508925235_22364 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Lamins are intermediate filament proteins that form a fibrous meshwork, called the nuclear lamina, between the inner nuclear membrane and peripheral heterochromatin of metazoan cells. The assembly and incorporation of lamin A/C into the lamina, as well as their various functions, are still not well understood. Here, we employed designed ankyrin repeat proteins (DARPins) as new experimental tools for lamin research. We screened for DARPins that specifically bound to lamin A/C, and interfered with lamin assembly in vitro and with incorporation of lamin A/C into the native lamina in living cells. The selected DARPins inhibited lamin assembly and delocalized A-type lamins to the nucleoplasm without modifying lamin expression levels or the amino acid sequence. Using these lamin binders, we demonstrate the importance of proper integration of lamin A/C into the lamina for nuclear mechanical properties and nuclear envelope integrity. Finally, our study provides evidence for cell-type-specific differences in lamin functions. |
| 536 | _ | _ | |a 312 - Functional and structural genomics (POF3-312) |0 G:(DE-HGF)POF3-312 |c POF3-312 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Lamin Type A |2 NLM Chemicals |
| 650 | _ | 7 | |a Lamin Type B |2 NLM Chemicals |
| 650 | _ | 7 | |a Lamins |2 NLM Chemicals |
| 700 | 1 | _ | |a Roschitzki-Voser, Heidi |b 1 |
| 700 | 1 | _ | |a Zbinden, Reto |b 2 |
| 700 | 1 | _ | |a Denais, Celine |b 3 |
| 700 | 1 | _ | |a Herrmann, Harald |0 P:(DE-He78)7892a89fee19b8e3912c7423d660765d |b 4 |u dkfz |
| 700 | 1 | _ | |a Lammerding, Jan |b 5 |
| 700 | 1 | _ | |a Grütter, Markus G |b 6 |
| 700 | 1 | _ | |a Medalia, Ohad |b 7 |
| 773 | _ | _ | |a 10.1242/jcs.171843 |g Vol. 128, no. 19, p. 3607 - 3620 |0 PERI:(DE-600)1483099-1 |n 19 |p 3607 - 3620 |t Journal of cell science |v 128 |y 2015 |x 1477-9137 |
| 909 | C | O | |o oai:inrepo02.dkfz.de:127890 |p VDB |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 4 |6 P:(DE-He78)7892a89fee19b8e3912c7423d660765d |
| 913 | 1 | _ | |a DE-HGF |l Krebsforschung |1 G:(DE-HGF)POF3-310 |0 G:(DE-HGF)POF3-312 |2 G:(DE-HGF)POF3-300 |v Functional and structural genomics |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |b Gesundheit |
| 914 | 1 | _ | |y 2015 |
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| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b J CELL SCI : 2015 |
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| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
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