%0 Journal Article
%A Laudato, Sara
%A Patil, Nitin Shaligram
%A Abba, Mohammed Lawan
%A Leupold, Joerg H
%A Benner, Axel
%A Gaiser, Timo
%A Marx, Alexander
%A Allgayer, Heike
%T P53-induced miR-30e-5p inhibits colorectal cancer invasion and metastasis by targeting ITGA6 and ITGB1.
%J International journal of cancer
%V 141
%N 9
%@ 0020-7136
%C Bognor Regis
%I Wiley-Liss
%M DKFZ-2017-04102
%P 1879 - 1890
%D 2017
%X The tumor suppressor P53 is a critical regulator of normal cellular homeostasis whose function is either distorted or lost in several cancer types including colorectal cancer (CRC). A small group of microRNAs have come to be recognized as essential mediators of P53 function. In a genome-wide systematic approach, we explored miRNAs that are substantially altered by P53 loss and found miR-30e to be the most significantly deregulated miRNA in P53-knockout human CRC cells. We identified miR-30e-5p to be a novel direct transcriptional target of P53 with gain and loss of function experiments revealing miR-30e-5p to be a significant regulator of tumor cell migration, invasion and in vivo metastasis mediated in part by integrins alpha-6 and beta-1 as novel targets. MiR-30e-5p also significantly reduced tumor cell proliferation by causing G1/S cell cycle arrest, which was achieved by inducing P21 and P27 expression. Finally, we found miR-30e-5p to be lost in resected CRC tumors as compared to normal colon tissues. Taken together, miR-30e-5p is a novel effector of P53-induced suppression of migration, invasion and metastasis.
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:28656629
%R 10.1002/ijc.30854
%U https://inrepo02.dkfz.de/record/128080