TY  - JOUR
AU  - Daaboul, Hamid E
AU  - Daher, Costantine F
AU  - Taleb, Robin I
AU  - Boulos, Joelle
AU  - Bodman-Smith, Kikki
AU  - Boukamp, Petra
AU  - Shebaby, Wassim N
AU  - Dagher, Carol
AU  - El-Sibai, Mirvat
AU  - Mroueh, Mohamad A
TI  - β-2-himachalen-6-ol protects against skin cancer development in vitro and in vivo.
JO  - Journal of pharmacy and pharmacology
VL  - 69
IS  - 11
SN  - 0022-3573
CY  - Wallingford, Oxon
PB  - Pharmaceutical Press64910
M1  - DKFZ-2017-04693
SP  - 1552 - 1564
PY  - 2017
AB  - Previous studies in our laboratory showed that Daucus carota oil extract (DCOE) possesses remarkable in-vitro anticancer activity and antitumour promoting effect against DMBA/TPA skin carcinogenesis in mice. Chemical analysis of DCOE led to the isolation of the β-2-himachalen-6-ol (HC), major sesquiterpene with a potent anticancer activity against various colon, breast, brain and skin cancer cells. This study investigated the anticancer activity of HC against invasive epidermal squamous cell carcinoma cells and evaluated its effect in a DMBA/TPA skin carcinogenesis Balb/c murine model.HaCaT-ras II-4 epidermal squamous cells were treated with HC (1, 5, 10, 25 and 50 μg/ml), and cell viability was evaluated with WST 1 assay kit. Cell cycle analysis was carried out by flow cytometry, and pro/anti-apoptotic proteins were measured using Western blot. The effect of topical and intraperitoneal (IP) treatment with HC in mice was assessed using the DMBA/TPA skin carcinogenesis model. Cisplatin (2.5 mg/kg; IP) was used as a positive control. Papilloma incidence, yield and volume were monitored, and isolated papillomas were assessed for their pro/anti-apoptotic proteins and morphology.β-2-himachalen-6-ol showed a dose-dependent decrease in cell survival with an IC50 and IC90 of 8 and 30 μg/ml, respectively. Flow cytometry analysis revealed that treatment with 10 μg/ml HC significantly increased the number of cells undergoing late apoptosis (28
LB  - PUB:(DE-HGF)16
C6  - pmid:28872682
DO  - DOI:10.1111/jphp.12796
UR  - https://inrepo02.dkfz.de/record/128678
ER  -