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000128686 0247_ $$2doi$$a10.1172/JCI94668
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000128686 037__ $$aDKFZ-2017-04701
000128686 041__ $$aeng
000128686 082__ $$a610
000128686 1001_ $$aKim, Jaeryung$$b0
000128686 245__ $$aImpaired angiopoietin/Tie2 signaling compromises Schlemm's canal integrity and induces glaucoma.
000128686 260__ $$aAnn Arbor, Mich.$$bASCJ$$c2017
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000128686 520__ $$aPrimary open-angle glaucoma (POAG) is often caused by elevated intraocular pressure (IOP), which arises due to increased resistance to aqueous humor outflow (AHO). Aqueous humor flows through Schlemm's canal (SC), a lymphatic-like vessel encircling the cornea, and via intercellular spaces of ciliary muscle cells. However, the mechanisms underlying increased AHO resistance are poorly understood. Here, we demonstrate that signaling between angiopoietin (Angpt) and the Angpt receptor Tie2, which is critical for SC formation, is also indispensable for maintaining SC integrity during adulthood. Deletion of Angpt1/Angpt2 or Tie2 in adult mice severely impaired SC integrity and transcytosis, leading to elevated IOP, retinal neuron damage, and impairment of retinal ganglion cell function, all hallmarks of POAG in humans. We found that SC integrity is maintained by interconnected and coordinated functions of Angpt-Tie2 signaling, AHO, and Prox1 activity. These functions diminish in the SC during aging, leading to impaired integrity and transcytosis. Intriguingly, Tie2 reactivation using a Tie2 agonistic antibody rescued the POAG phenotype in Angpt1/Angpt2-deficient mice and rejuvenated the SC in aged mice. These results indicate that the Angpt-Tie2 system is essential for SC integrity. The impairment of this system underlies POAG-associated pathogenesis, supporting the possibility that Tie2 agonists could be a therapeutic option for glaucoma.
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000128686 650_7 $$2NLM Chemicals$$aANGPT1 protein, human
000128686 650_7 $$2NLM Chemicals$$aANGPT2 protein, human
000128686 650_7 $$2NLM Chemicals$$aAngiopoietin-1
000128686 650_7 $$2NLM Chemicals$$aAngiopoietin-2
000128686 650_7 $$2NLM Chemicals$$aAngpt1 protein, mouse
000128686 650_7 $$2NLM Chemicals$$aHomeodomain Proteins
000128686 650_7 $$2NLM Chemicals$$aTumor Suppressor Proteins
000128686 650_7 $$2NLM Chemicals$$aprospero-related homeobox 1 protein
000128686 7001_ $$aPark, Dae-Young$$b1
000128686 7001_ $$aBae, Hosung$$b2
000128686 7001_ $$aPark, Do Young$$b3
000128686 7001_ $$aKim, Dongkyu$$b4
000128686 7001_ $$aLee, Choong-Kun$$b5
000128686 7001_ $$aSong, Sukhyun$$b6
000128686 7001_ $$aChung, Tae-Young$$b7
000128686 7001_ $$aLim, Dong Hui$$b8
000128686 7001_ $$aKubota, Yoshiaki$$b9
000128686 7001_ $$aHong, Young-Kwon$$b10
000128686 7001_ $$aHe, Yulong$$b11
000128686 7001_ $$0P:(DE-He78)2e92d0ae281932fc7347d819fec36b0b$$aAugustin, Hellmut$$b12$$udkfz
000128686 7001_ $$aOliver, Guillermo$$b13
000128686 7001_ $$aKoh, Gou Young$$b14
000128686 773__ $$0PERI:(DE-600)2018375-6$$a10.1172/JCI94668$$gVol. 127, no. 10, p. 3877 - 3896$$n10$$p3877 - 3896$$tThe @journal of clinical investigation$$v127$$x1558-8238$$y2017
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000128686 9141_ $$y2017
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