%0 Journal Article
%A Kempf, Anissa
%A Boda, Enrica
%A Kwok, Jessica C F
%A Fritz, Rafael
%A Grande, Valentina
%A Kaelin, Andrea M
%A Ristic, Zorica
%A Schmandke, Andre
%A Schmandke, Antonio
%A Tews, Bjoern
%A Fawcett, James W
%A Pertz, Olivier
%A Buffo, Annalisa
%A Schwab, Martin E
%T Control of Cell Shape, Neurite Outgrowth, and Migration by a Nogo-A/HSPG Interaction.
%J Developmental cell
%V 43
%N 1
%@ 1534-5807
%C Cambridge, Mass.
%I Cell Press
%M DKFZ-2017-05182
%P 24 - 34.e5
%D 2017
%X Heparan sulfate proteoglycans (HSPGs) critically modulate adhesion-, growth-, and migration-related processes. Here, we show that the transmembrane protein, Nogo-A, inhibits neurite outgrowth and cell spreading in neurons and Nogo-A-responsive cell lines via HSPGs. The extracellular, active 180 amino acid Nogo-A region, named Nogo-A-Δ20, binds to heparin and brain-derived heparan sulfate glycosaminoglycans (GAGs) but not to the closely related chondroitin sulfate GAGs. HSPGs are required for Nogo-A-Δ20-induced inhibition of adhesion, cell spreading, and neurite outgrowth, as well as for RhoA activation. Surprisingly, we show that Nogo-A-Δ20 can act via HSPGs independently of its receptor, Sphingosine-1-Phosphate receptor 2 (S1PR2). We thereby identify the HSPG family members syndecan-3 and syndecan-4 as functional receptors for Nogo-A-Δ20. Finally, we show in explant cultures ex vivo that Nogo-A-Δ20 promotes the migration of neuroblasts via HSPGs but not S1PR2.
%K Carrier Proteins (NLM Chemicals)
%K Heparan Sulfate Proteoglycans (NLM Chemicals)
%K Nogo Proteins (NLM Chemicals)
%K Proteoglycans (NLM Chemicals)
%K Receptors, Lysosphingolipid (NLM Chemicals)
%K Heparitin Sulfate (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:28943240
%R 10.1016/j.devcel.2017.08.014
%U https://inrepo02.dkfz.de/record/129177