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000129179 1001_ $$0P:(DE-He78)f89c856740c99008f4fbc1efa501d4f9$$aLarribère, Lionel$$b0$$eFirst author
000129179 245__ $$aAn RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs.
000129179 260__ $$aMaryland Heights, MO$$bCell Press$$c2017
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000129179 520__ $$aMolecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput imaging of early epithelial cells, we identified candidate kinases involved in their differentiation from hiPSCs. Among these, we found HIPK4 to be an important inhibitor of this process. Indeed, its silencing increased the amount of generated skin epithelial precursors at an early time point, increased the amount of generated keratinocytes at a later time point, and improved growth and differentiation of organotypic cultures, allowing for the formation of a denser basal layer and stratification with the expression of several keratins. Our data bring substantial input regarding regulation of human skin epithelial differentiation and for improving differentiation protocols from pluripotent stem cells.
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000129179 7001_ $$0P:(DE-HGF)0$$aGalach, Marta$$b1$$eFirst author
000129179 7001_ $$0P:(DE-He78)5f0b1c9863f44d0695555ee3c22b9758$$aNovak, Daniel$$b2
000129179 7001_ $$0P:(DE-HGF)0$$aArévalo, Karla$$b3
000129179 7001_ $$0P:(DE-HGF)0$$aVolz, Hans Christian$$b4
000129179 7001_ $$0P:(DE-He78)2fbb71b04b02738300427866d6e3181a$$aStark, Hans-Jürgen$$b5
000129179 7001_ $$0P:(DE-He78)c1895aa471c7ac9c7173045464b69b31$$aBoukamp, Petra$$b6
000129179 7001_ $$0P:(DE-He78)3c0da8e3caa2aa50cad85152aa0465ad$$aBoutros, Michael$$b7
000129179 7001_ $$0P:(DE-He78)a229f7724466e7efadf4a1ace1ff8af3$$aUtikal, Jochen$$b8$$eLast author
000129179 773__ $$0PERI:(DE-600)2720528-9$$a10.1016/j.stemcr.2017.08.023$$gVol. 9, no. 4, p. 1234 - 1245$$n4$$p1234 - 1245$$tStem cell reports$$v9$$x2213-6711$$y2017
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