TY  - JOUR
AU  - Larribère, Lionel
AU  - Galach, Marta
AU  - Novak, Daniel
AU  - Arévalo, Karla
AU  - Volz, Hans Christian
AU  - Stark, Hans-Jürgen
AU  - Boukamp, Petra
AU  - Boutros, Michael
AU  - Utikal, Jochen
TI  - An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs.
JO  - Stem cell reports
VL  - 9
IS  - 4
SN  - 2213-6711
CY  - Maryland Heights, MO
PB  - Cell Press
M1  - DKFZ-2017-05184
SP  - 1234 - 1245
PY  - 2017
AB  - Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput imaging of early epithelial cells, we identified candidate kinases involved in their differentiation from hiPSCs. Among these, we found HIPK4 to be an important inhibitor of this process. Indeed, its silencing increased the amount of generated skin epithelial precursors at an early time point, increased the amount of generated keratinocytes at a later time point, and improved growth and differentiation of organotypic cultures, allowing for the formation of a denser basal layer and stratification with the expression of several keratins. Our data bring substantial input regarding regulation of human skin epithelial differentiation and for improving differentiation protocols from pluripotent stem cells.
LB  - PUB:(DE-HGF)16
C6  - pmid:28966120
C2  - pmc:PMC5639458
DO  - DOI:10.1016/j.stemcr.2017.08.023
UR  - https://inrepo02.dkfz.de/record/129179
ER  -