guest ::
| Home > Publications database > An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. > print |
| Home > Publications database > An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. > print |
| 001 | 129179 | ||
| 005 | 20240228145548.0 | ||
| 024 | 7 | _ | |a 10.1016/j.stemcr.2017.08.023 |2 doi |
| 024 | 7 | _ | |a pmid:28966120 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC5639458 |2 pmc |
| 024 | 7 | _ | |a altmetric:26774103 |2 altmetric |
| 037 | _ | _ | |a DKFZ-2017-05184 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Larribère, Lionel |0 P:(DE-He78)f89c856740c99008f4fbc1efa501d4f9 |b 0 |e First author |
| 245 | _ | _ | |a An RNAi Screen Reveals an Essential Role for HIPK4 in Human Skin Epithelial Differentiation from iPSCs. |
| 260 | _ | _ | |a Maryland Heights, MO |c 2017 |b Cell Press |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1660813716_9237 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Molecular mechanisms responsible for the development of human skin epithelial cells are incompletely understood. As a consequence, the efficiency to establish a pure skin epithelial cell population from human induced pluripotent stem cells (hiPSCs) remains poor. Using an approach including RNAi and high-throughput imaging of early epithelial cells, we identified candidate kinases involved in their differentiation from hiPSCs. Among these, we found HIPK4 to be an important inhibitor of this process. Indeed, its silencing increased the amount of generated skin epithelial precursors at an early time point, increased the amount of generated keratinocytes at a later time point, and improved growth and differentiation of organotypic cultures, allowing for the formation of a denser basal layer and stratification with the expression of several keratins. Our data bring substantial input regarding regulation of human skin epithelial differentiation and for improving differentiation protocols from pluripotent stem cells. |
| 536 | _ | _ | |a 317 - Translational cancer research (POF3-317) |0 G:(DE-HGF)POF3-317 |c POF3-317 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 700 | 1 | _ | |a Galach, Marta |0 P:(DE-HGF)0 |b 1 |e First author |
| 700 | 1 | _ | |a Novak, Daniel |0 P:(DE-He78)5f0b1c9863f44d0695555ee3c22b9758 |b 2 |
| 700 | 1 | _ | |a Arévalo, Karla |0 P:(DE-HGF)0 |b 3 |
| 700 | 1 | _ | |a Volz, Hans Christian |0 P:(DE-HGF)0 |b 4 |
| 700 | 1 | _ | |a Stark, Hans-Jürgen |0 P:(DE-He78)2fbb71b04b02738300427866d6e3181a |b 5 |
| 700 | 1 | _ | |a Boukamp, Petra |0 P:(DE-He78)c1895aa471c7ac9c7173045464b69b31 |b 6 |
| 700 | 1 | _ | |a Boutros, Michael |0 P:(DE-He78)3c0da8e3caa2aa50cad85152aa0465ad |b 7 |
| 700 | 1 | _ | |a Utikal, Jochen |0 P:(DE-He78)a229f7724466e7efadf4a1ace1ff8af3 |b 8 |e Last author |
| 773 | _ | _ | |a 10.1016/j.stemcr.2017.08.023 |g Vol. 9, no. 4, p. 1234 - 1245 |0 PERI:(DE-600)2720528-9 |n 4 |p 1234 - 1245 |t Stem cell reports |v 9 |y 2017 |x 2213-6711 |
| 909 | C | O | |p VDB |o oai:inrepo02.dkfz.de:129179 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 0 |6 P:(DE-He78)f89c856740c99008f4fbc1efa501d4f9 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 1 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 2 |6 P:(DE-He78)5f0b1c9863f44d0695555ee3c22b9758 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 3 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 4 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 5 |6 P:(DE-He78)2fbb71b04b02738300427866d6e3181a |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 6 |6 P:(DE-He78)c1895aa471c7ac9c7173045464b69b31 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 7 |6 P:(DE-He78)3c0da8e3caa2aa50cad85152aa0465ad |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 8 |6 P:(DE-He78)a229f7724466e7efadf4a1ace1ff8af3 |
| 913 | 1 | _ | |a DE-HGF |b Gesundheit |l Krebsforschung |1 G:(DE-HGF)POF3-310 |0 G:(DE-HGF)POF3-317 |3 G:(DE-HGF)POF3 |2 G:(DE-HGF)POF3-300 |4 G:(DE-HGF)POF |v Translational cancer research |x 0 |
| 914 | 1 | _ | |y 2017 |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b STEM CELL REP : 2015 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0501 |2 StatID |b DOAJ Seal |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0500 |2 StatID |b DOAJ |
| 915 | _ | _ | |a Creative Commons Attribution-NonCommercial CC BY-NC (No Version) |0 LIC:(DE-HGF)CCBYNCNV |2 V:(DE-HGF) |b DOAJ |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |
| 915 | _ | _ | |a IF >= 5 |0 StatID:(DE-HGF)9905 |2 StatID |b STEM CELL REP : 2015 |
| 920 | 1 | _ | |0 I:(DE-He78)G300-20160331 |k G300 |l KKE Dermatoonkologie |x 0 |
| 920 | 1 | _ | |0 I:(DE-He78)B110-20160331 |k B110 |l B110 Signalwege funktionelle Genomik |x 1 |
| 920 | 1 | _ | |0 I:(DE-He78)A110-20160331 |k A110 |l Genetik der Hautkarcinogenese |x 2 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-He78)G300-20160331 |
| 980 | _ | _ | |a I:(DE-He78)B110-20160331 |
| 980 | _ | _ | |a I:(DE-He78)A110-20160331 |
| 980 | _ | _ | |a UNRESTRICTED |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|