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000130228 0247_ $$2doi$$a10.1186/s12915-016-0247-4
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000130228 041__ $$aeng
000130228 082__ $$a570
000130228 1001_ $$aNavarro, Gemma$$b0
000130228 245__ $$aQuaternary structure of a G-protein-coupled receptor heterotetramer in complex with Gi and Gs.
000130228 260__ $$aBerlin$$bSpringer$$c2016
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000130228 520__ $$aG-protein-coupled receptors (GPCRs), in the form of monomers or homodimers that bind heterotrimeric G proteins, are fundamental in the transfer of extracellular stimuli to intracellular signaling pathways. Different GPCRs may also interact to form heteromers that are novel signaling units. Despite the exponential growth in the number of solved GPCR crystal structures, the structural properties of heteromers remain unknown.We used single-particle tracking experiments in cells expressing functional adenosine A1-A2A receptors fused to fluorescent proteins to show the loss of Brownian movement of the A1 receptor in the presence of the A2A receptor, and a preponderance of cell surface 2:2 receptor heteromers (dimer of dimers). Using computer modeling, aided by bioluminescence resonance energy transfer assays to monitor receptor homomerization and heteromerization and G-protein coupling, we predict the interacting interfaces and propose a quaternary structure of the GPCR tetramer in complex with two G proteins.The combination of results points to a molecular architecture formed by a rhombus-shaped heterotetramer, which is bound to two different interacting heterotrimeric G proteins (Gi and Gs). These novel results constitute an important advance in understanding the molecular intricacies involved in GPCR function.
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000130228 650_7 $$2NLM Chemicals$$aReceptors, Purinergic P1
000130228 650_7 $$0EC 3.6.5.1$$2NLM Chemicals$$aHeterotrimeric GTP-Binding Proteins
000130228 7001_ $$aCordomí, Arnau$$b1
000130228 7001_ $$0P:(DE-HGF)0$$aZelman-Femiak, Monika$$b2
000130228 7001_ $$aBrugarolas, Marc$$b3
000130228 7001_ $$aMoreno, Estefania$$b4
000130228 7001_ $$aAguinaga, David$$b5
000130228 7001_ $$aPerez-Benito, Laura$$b6
000130228 7001_ $$aCortés, Antoni$$b7
000130228 7001_ $$aCasadó, Vicent$$b8
000130228 7001_ $$aMallol, Josefa$$b9
000130228 7001_ $$aCanela, Enric I$$b10
000130228 7001_ $$aLluís, Carme$$b11
000130228 7001_ $$aPardo, Leonardo$$b12
000130228 7001_ $$0P:(DE-HGF)0$$aGarcía-Sáez, Ana J$$b13
000130228 7001_ $$aMcCormick, Peter J$$b14
000130228 7001_ $$aFranco, Rafael$$b15
000130228 773__ $$0PERI:(DE-600)2133020-7$$a10.1186/s12915-016-0247-4$$gVol. 14, no. 1, p. 26$$n1$$p26$$tBMC biology$$v14$$x1741-7007$$y2016
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