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@ARTICLE{Pecqueux:130302,
      author       = {M. Pecqueux and I. Liebetrau and W. Werft$^*$ and H.
                      Dienemann and T. Muley and J. Pfannschmidt and B. Müssle
                      and N. Rahbari and S. Schölch and M. W. Büchler and J.
                      Weitz and C. Reissfelder and C. Kahlert},
      title        = {{A} {C}omprehensive {M}icro{RNA} {E}xpression {P}rofile of
                      {L}iver and {L}ung {M}etastases of {C}olorectal {C}ancer
                      with {T}heir {C}orresponding {H}ost {T}issue and {I}ts
                      {P}rognostic {I}mpact on {S}urvival.},
      journal      = {International journal of molecular sciences},
      volume       = {17},
      number       = {10},
      issn         = {1422-0067},
      address      = {Basel},
      publisher    = {Molecular Diversity Preservation International},
      reportid     = {DKFZ-2017-05381},
      pages        = {1755 -},
      year         = {2016},
      abstract     = {MicroRNAs are small non-coding RNAs with a length of 18-25
                      nucleotides. They can regulate tumor invasion and metastasis
                      by changing the expression and translation of their target
                      mRNAs. Their expression is substantially altered in
                      colorectal cancer cells as well as in the adjacent
                      tumor-associated stroma. Both of these compartments have a
                      mutual influence on tumor progression. In the development of
                      metastases, cancer cells initially interact with the host
                      tissue. Therefore, compartment-specific expression
                      signatures of these three locations-tumor, associated
                      stroma, and host tissue-can provide new insights into the
                      complex tumor biology of colorectal cancer. Frozen tissue
                      samples of colorectal liver (n = 25) and lung metastases (n
                      = 24) were laser microdissected to separate tumor cells and
                      the adjacent tumor-associated stroma cells. Additionally,
                      normal lung and liver tissue was collected from the same
                      patients. We performed a microarray analysis in four
                      randomly selected liver metastases and four randomly
                      selected lung metastases, analyzing a total of 939 human
                      miRNAs. miRNAs with a significant change >2-fold between the
                      tumor, tumor stroma, and host tissue were analyzed in all
                      samples using RT-qPCR (11 miRNAs) and correlated with the
                      clinical data. We found a differential expression of several
                      miRNAs between the tumor, the tumor-associated stroma, and
                      the host tissue compartment. When comparing liver and lung
                      metastases, miR-194 showed a 1.5-fold; miR-125, miR-127, and
                      miR-192 showed a 2.5-fold; miR-19 and miR-215 a 3-fold;
                      miR-145, miR-199-3, and miR-429 a 5-fold; miR-21 a 7-fold;
                      and, finally, miR-199-5 a 12.5-fold downregulation in liver
                      metastases compared to lung metastases. Furthermore miR-19,
                      miR-125, miR-127, miR-192, miR-194, miR-199-5, and miR-215
                      showed a significant upregulation in the normal liver tissue
                      compared to the normal lung tissue. Univariate analysis
                      identified an association of poor survival with the
                      expression of miR-125 (p = 0.05), miR-127 (p = 0.001),
                      miR-145 (p = 0.005), miR-192 (p = 0.015), miR-194 (0.003),
                      miR-199-5 (p = 0.008), miR-215 (p < 0.001), and miR-429 (p =
                      0.03) in the host liver tissue of the liver metastases.
                      Colorectal liver and lung metastases have a unique miRNA
                      expression profile. miRNA expression in the host tissue of
                      colorectal liver metastases seems to be able to influence
                      tumor progression and survival. These findings can be used
                      in the development of tailored therapies.},
      keywords     = {MicroRNAs (NLM Chemicals)},
      cin          = {C060},
      ddc          = {570},
      cid          = {I:(DE-He78)C060-20160331},
      pnm          = {313 - Cancer risk factors and prevention (POF3-313)},
      pid          = {G:(DE-HGF)POF3-313},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:27775664},
      pmc          = {pmc:PMC5085780},
      doi          = {10.3390/ijms17101755},
      url          = {https://inrepo02.dkfz.de/record/130302},
}