%0 Journal Article
%A Scott-Browne, James P
%A López-Moyado, Isaac F
%A Trifari, Sara
%A Wong, Victor
%A Chavez, Lukas
%A Rao, Anjana
%A Pereira, Renata M
%T Dynamic Changes in Chromatin Accessibility Occur in CD8(+) T Cells Responding to Viral Infection.
%J Immunity
%V 45
%N 6
%@ 1074-7613
%C [Cambridge, Mass.]
%I Cell Press
%M DKFZ-2017-05615
%P 1327 - 1340
%D 2016
%X In response to acute infection, naive CD8(+) T cells expand, differentiate into effector cells, and then contract to a long-lived pool of memory cells after pathogen clearance. During chronic infections or in tumors, CD8(+) T cells acquire an 'exhausted' phenotype. Here we present genome-wide comparisons of chromatin accessibility and gene expression from endogenous CD8(+) T cells responding to acute and chronic viral infection using ATAC-seq and RNA-seq techniques. Acquisition of effector, memory, or exhausted phenotypes was associated with stable changes in chromatin accessibility away from the naive T cell state. Regions differentially accessible between functional subsets in vivo were enriched for binding sites of transcription factors known to regulate these subsets, including E2A, BATF, IRF4, T-bet, and TCF1. Exhaustion-specific accessible regions were enriched for consensus binding sites for NFAT and Nr4a family members, indicating that chronic stimulation confers a unique accessibility profile on exhausted cells.
%K Chromatin (NLM Chemicals)
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:27939672
%2 pmc:PMC5214519
%R 10.1016/j.immuni.2016.10.028
%U https://inrepo02.dkfz.de/record/130536