TY  - JOUR
AU  - Scott-Browne, James P
AU  - López-Moyado, Isaac F
AU  - Trifari, Sara
AU  - Wong, Victor
AU  - Chavez, Lukas
AU  - Rao, Anjana
AU  - Pereira, Renata M
TI  - Dynamic Changes in Chromatin Accessibility Occur in CD8(+) T Cells Responding to Viral Infection.
JO  - Immunity
VL  - 45
IS  - 6
SN  - 1074-7613
CY  - [Cambridge, Mass.]
PB  - Cell Press
M1  - DKFZ-2017-05615
SP  - 1327 - 1340
PY  - 2016
AB  - In response to acute infection, naive CD8(+) T cells expand, differentiate into effector cells, and then contract to a long-lived pool of memory cells after pathogen clearance. During chronic infections or in tumors, CD8(+) T cells acquire an 'exhausted' phenotype. Here we present genome-wide comparisons of chromatin accessibility and gene expression from endogenous CD8(+) T cells responding to acute and chronic viral infection using ATAC-seq and RNA-seq techniques. Acquisition of effector, memory, or exhausted phenotypes was associated with stable changes in chromatin accessibility away from the naive T cell state. Regions differentially accessible between functional subsets in vivo were enriched for binding sites of transcription factors known to regulate these subsets, including E2A, BATF, IRF4, T-bet, and TCF1. Exhaustion-specific accessible regions were enriched for consensus binding sites for NFAT and Nr4a family members, indicating that chronic stimulation confers a unique accessibility profile on exhausted cells.
KW  - Chromatin (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:27939672
C2  - pmc:PMC5214519
DO  - DOI:10.1016/j.immuni.2016.10.028
UR  - https://inrepo02.dkfz.de/record/130536
ER  -