TY  - JOUR
AU  - Sharma, Sahil
AU  - Poetz, Fabian
AU  - Bruer, Marius
AU  - Ly-Hartig, Thi Bach Nga
AU  - Schott, Johanna
AU  - Séraphin, Bertrand
AU  - Stoecklin, Georg
TI  - Acetylation-Dependent Control of Global Poly(A) RNA Degradation by CBP/p300 and HDAC1/2.
JO  - Molecular cell
VL  - 63
IS  - 6
SN  - 1097-2765
CY  - [Cambridge, Mass.]
PB  - Cell Press
M1  - DKFZ-2017-05631
SP  - 927 - 938
PY  - 2016
AB  - Acetylation of histones and transcription-related factors is known to exert epigenetic and transcriptional control of gene expression. Here we report that histone acetyltransferases (HATs) and histone deacetylases (HDACs) also regulate gene expression at the posttranscriptional level by controlling poly(A) RNA stability. Inhibition of HDAC1 and HDAC2 induces massive and widespread degradation of normally stable poly(A) RNA in mammalian and Drosophila cells. Acetylation-induced RNA decay depends on the HATs p300 and CBP, which acetylate the exoribonuclease CAF1a, a catalytic subunit of the CCR4-CAF1-NOT deadenlyase complex and thereby contribute to accelerating poly(A) RNA degradation. Taking adipocyte differentiation as a model, we observe global stabilization of poly(A) RNA during differentiation, concomitant with loss of CBP/p300 expression. Our study uncovers reversible acetylation as a fundamental switch by which HATs and HDACs control the overall turnover of poly(A) RNA.
KW  - CNOT8 protein, human (NLM Chemicals)
KW  - NR4A2 protein, human (NLM Chemicals)
KW  - Nuclear Receptor Subfamily 4, Group A, Member 2 (NLM Chemicals)
KW  - RNA, Messenger (NLM Chemicals)
KW  - Transcription Factors (NLM Chemicals)
KW  - Poly A (NLM Chemicals)
KW  - p300-CBP Transcription Factors (NLM Chemicals)
KW  - p300-CBP-associated factor (NLM Chemicals)
KW  - HDAC1 protein, human (NLM Chemicals)
KW  - HDAC2 protein, human (NLM Chemicals)
KW  - Histone Deacetylase 1 (NLM Chemicals)
KW  - Histone Deacetylase 2 (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:27635759
DO  - DOI:10.1016/j.molcel.2016.08.030
UR  - https://inrepo02.dkfz.de/record/130552
ER  -