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@ARTICLE{Staufer:130598,
author = {O. Staufer$^*$ and S. Weber and C. P. Bengtson and H.
Bading and J. P. Spatz and A. Rustom},
title = {{F}unctional fusion of living systems with synthetic
electrode interfaces.},
journal = {Beilstein journal of nanotechnology},
volume = {7},
issn = {2190-4286},
address = {Frankfurt, M.},
publisher = {Beilstein-Institut zur Förderung der Chemischen
Wissenschaften},
reportid = {DKFZ-2017-05676},
pages = {296 - 301},
year = {2016},
abstract = {The functional fusion of 'living' biomaterial (such as
cells) with synthetic systems has developed into a principal
ambition for various scientific disciplines. In particular,
emerging fields such as bionics and nanomedicine integrate
advanced nanomaterials with biomolecules, cells and
organisms in order to develop novel strategies for
applications, including energy production or real-time
diagnostics utilizing biomolecular machineries 'perfected'
during billion years of evolution. To date, hardware-wetware
interfaces that sample or modulate bioelectric potentials,
such as neuroprostheses or implantable energy harvesters,
are mostly based on microelectrodes brought into the closest
possible contact with the targeted cells. Recently, the
possibility of using electrochemical gradients of the inner
ear for technical applications was demonstrated using
implanted electrodes, where 1.12 nW of electrical power was
harvested from the guinea pig endocochlear potential for up
to 5 h (Mercier, P.; Lysaght, A.; Bandyopadhyay, S.;
Chandrakasan, A.; Stankovic, K. Nat. Biotech. 2012, 30,
1240-1243). More recent approaches employ nanowires (NWs)
able to penetrate the cellular membrane and to record extra-
and intracellular electrical signals, in some cases with
subcellular resolution (Spira, M.; Hai, A. Nat. Nano. 2013,
8, 83-94). Such techniques include nanoelectric scaffolds
containing free-standing silicon NWs (Robinson, J. T.;
Jorgolli, M.; Shalek, A. K.; Yoon, M. H.; Gertner, R. S.;
Park, H. Nat Nanotechnol. 2012, 10, 180-184) or NW
field-effect transistors (Qing, Q.; Jiang, Z.; Xu, L.; Gao,
R.; Mai, L.; Lieber, C. Nat. Nano. 2013, 9, 142-147),
vertically aligned gallium phosphide NWs (Hällström, W.;
Mårtensson, T.; Prinz, C.; Gustavsson, P.; Montelius, L.;
Samuelson, L.; Kanje, M. Nano Lett. 2007, 7, 2960-2965) or
individually contacted, electrically active carbon
nanofibers. The latter of these approaches is capable of
recording electrical responses from oxidative events
occurring in intercellular regions of neuronal cultures
(Zhang, D.; Rand, E.; Marsh, M.; Andrews, R.; Lee, K.;
Meyyappan, M.; Koehne, J. Mol. Neurobiol. 2013, 48,
380-385). Employing monocrystalline gold, nanoelectrode
interfaces, we have now achieved stable, functional access
to the electrochemical machinery of individual Physarum
polycephalum slime mold cells. We demonstrate the
'symbionic' union, allowing for electrophysiological
measurements, functioning as autonomous sensors and capable
of producing nanowatts of electric power. This represents a
further step towards the future development of
groundbreaking, cell-based technologies, such as bionic
sensory systems or miniaturized energy sources to power
various devices, or even 'intelligent implants', constantly
refueled by their surrounding nutrients.},
cin = {M120},
ddc = {620},
cid = {I:(DE-He78)M120-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:26977386},
pmc = {pmc:PMC4778514},
doi = {10.3762/bjnano.7.27},
url = {https://inrepo02.dkfz.de/record/130598},
}