TY  - JOUR
AU  - Tang, Qiuqiong
AU  - Holland-Letz, Tim
AU  - Slynko, Alla
AU  - Cuk, Katarina
AU  - Marme, Frederik
AU  - Schott, Sarah
AU  - Heil, Jörg
AU  - Qu, Bin
AU  - Golatta, Michael
AU  - Bewerunge-Hudler, Melanie
AU  - Sutter, Christian
AU  - Surowy, Harald
AU  - Wappenschmidt, Barbara
AU  - Schmutzler, Rita
AU  - Hoth, Markus
AU  - Bugert, Peter
AU  - Bartram, Claus R
AU  - Sohn, Christof
AU  - Schneeweiss, Andreas
AU  - Yang, Rongxi
AU  - Burwinkel, Barbara
TI  - DNA methylation array analysis identifies breast cancer associated RPTOR, MGRN1 and RAPSN hypomethylation in peripheral blood DNA.
JO  - OncoTarget
VL  - 7
IS  - 39
SN  - 1949-2553
CY  - [S.l.]
PB  - Impact Journals LLC
M1  - DKFZ-2017-05728
SP  - 64191 - 64202
PY  - 2016
AB  - DNA methylation changes in peripheral blood DNA have been shown to be associated with solid tumors. We sought to identify methylation alterations in whole blood DNA that are associated with breast cancer (BC). Epigenome-wide DNA methylation profiling on blood DNA from BC cases and healthy controls was performed by applying Infinium HumanMethylation450K BeadChips. Promising CpG sites were selected and validated in three independent larger sample cohorts via MassARRAY EpiTyper assays. CpG sites located in three genes (cg06418238 in RPTOR, cg00736299 in MGRN1 and cg27466532 in RAPSN), which showed significant hypomethylation in BC patients compared to healthy controls in the discovery cohort (p < 1.00 x 10-6) were selected and successfully validated in three independent cohorts (validation I, n =211; validation II, n=378; validation III, n=520). The observed methylation differences are likely not cell-type specific, as the differences were only seen in whole blood, but not in specific sub cell-types of leucocytes. Moreover, we observed in quartile analysis that women in the lower methylation quartiles of these three loci had higher ORs than women in the higher quartiles. The combined AUC of three loci was 0.79 (95
LB  - PUB:(DE-HGF)16
C6  - pmid:27577081
C2  - pmc:PMC5325435
DO  - DOI:10.18632/oncotarget.11640
UR  - https://inrepo02.dkfz.de/record/130650
ER  -