%0 Journal Article
%A Panwalkar, Pooja
%A Clark, Jonathan
%A Ramaswamy, Vijay
%A Hawes, Debra
%A Yang, Fusheng
%A Dunham, Christopher
%A Yip, Stephen
%A Hukin, Juliette
%A Sun, Yilun
%A Schipper, Matthew J
%A Chavez, Lukas
%A Margol, Ashley
%A Pekmezci, Melike
%A Chung, Chan
%A Banda, Adam
%A Bayliss, Jill M
%A Curry, Sarah J
%A Santi, Mariarita
%A Rodriguez, Fausto J
%A Snuderl, Matija
%A Karajannis, Matthias A
%A Saratsis, Amanda M
%A Horbinski, Craig M
%A Carret, Anne-Sophie
%A Wilson, Beverly
%A Johnston, Donna
%A Lafay-Cousin, Lucie
%A Zelcer, Shayna
%A Eisenstat, David
%A Silva, Marianna
%A Scheinemann, Katrin
%A Jabado, Nada
%A McNeely, P Daniel
%A Kool, Marcel
%A Pfister, Stefan
%A Taylor, Michael D
%A Hawkins, Cynthia
%A Korshunov, Andrey
%A Judkins, Alexander R
%A Venneti, Sriram
%T Immunohistochemical analysis of H3K27me3 demonstrates global reduction in group-A childhood posterior fossa ependymoma and is a powerful predictor of outcome.
%J Acta neuropathologica
%V 134
%N 5
%@ 1432-0533
%C Berlin
%I Springer
%M DKFZ-2017-05969
%P 705 - 714
%D 2017
%X Posterior fossa ependymomas (EPN_PF) in children comprise two morphologically identical, but biologically distinct tumor entities. Group-A (EPN_PFA) tumors have a poor prognosis and require intensive therapy. In contrast, group-B tumors (EPN_PFB) exhibit excellent prognosis and the current consensus opinion recommends future clinical trials to test the possibility of treatment de-escalation in these patients. Therefore, distinguishing these two tumor subtypes is critical. EPN_PFA and EPN_PFB can be distinguished based on DNA methylation signatures, but these assays are not routinely available. We have previously shown that a subset of poorly prognostic childhood EPN_PF exhibits global reduction in H3K27me3. Therefore, we set out to determine whether a simple immunohistochemical assay for H3K27me3 could be used to segregate EPN_PFA from EPN_PFB tumors. We assembled a cohort of 230 childhood ependymomas and H3K27me3 immunohistochemistry was assessed as positive or negative in a blinded manner. H3K27me3 staining results were compared with DNA methylation-based subgroup information available in 112 samples [EPN_PFA (n = 72) and EPN_PFB tumors (n = 40)]. H3K27me3 staining was globally reduced in EPN_PFA tumors and immunohistochemistry showed 99
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:28733933
%2 pmc:PMC5647236
%R 10.1007/s00401-017-1752-4
%U https://inrepo02.dkfz.de/record/130893