TY  - JOUR
AU  - Panwalkar, Pooja
AU  - Clark, Jonathan
AU  - Ramaswamy, Vijay
AU  - Hawes, Debra
AU  - Yang, Fusheng
AU  - Dunham, Christopher
AU  - Yip, Stephen
AU  - Hukin, Juliette
AU  - Sun, Yilun
AU  - Schipper, Matthew J
AU  - Chavez, Lukas
AU  - Margol, Ashley
AU  - Pekmezci, Melike
AU  - Chung, Chan
AU  - Banda, Adam
AU  - Bayliss, Jill M
AU  - Curry, Sarah J
AU  - Santi, Mariarita
AU  - Rodriguez, Fausto J
AU  - Snuderl, Matija
AU  - Karajannis, Matthias A
AU  - Saratsis, Amanda M
AU  - Horbinski, Craig M
AU  - Carret, Anne-Sophie
AU  - Wilson, Beverly
AU  - Johnston, Donna
AU  - Lafay-Cousin, Lucie
AU  - Zelcer, Shayna
AU  - Eisenstat, David
AU  - Silva, Marianna
AU  - Scheinemann, Katrin
AU  - Jabado, Nada
AU  - McNeely, P Daniel
AU  - Kool, Marcel
AU  - Pfister, Stefan
AU  - Taylor, Michael D
AU  - Hawkins, Cynthia
AU  - Korshunov, Andrey
AU  - Judkins, Alexander R
AU  - Venneti, Sriram
TI  - Immunohistochemical analysis of H3K27me3 demonstrates global reduction in group-A childhood posterior fossa ependymoma and is a powerful predictor of outcome.
JO  - Acta neuropathologica
VL  - 134
IS  - 5
SN  - 1432-0533
CY  - Berlin
PB  - Springer
M1  - DKFZ-2017-05969
SP  - 705 - 714
PY  - 2017
AB  - Posterior fossa ependymomas (EPN_PF) in children comprise two morphologically identical, but biologically distinct tumor entities. Group-A (EPN_PFA) tumors have a poor prognosis and require intensive therapy. In contrast, group-B tumors (EPN_PFB) exhibit excellent prognosis and the current consensus opinion recommends future clinical trials to test the possibility of treatment de-escalation in these patients. Therefore, distinguishing these two tumor subtypes is critical. EPN_PFA and EPN_PFB can be distinguished based on DNA methylation signatures, but these assays are not routinely available. We have previously shown that a subset of poorly prognostic childhood EPN_PF exhibits global reduction in H3K27me3. Therefore, we set out to determine whether a simple immunohistochemical assay for H3K27me3 could be used to segregate EPN_PFA from EPN_PFB tumors. We assembled a cohort of 230 childhood ependymomas and H3K27me3 immunohistochemistry was assessed as positive or negative in a blinded manner. H3K27me3 staining results were compared with DNA methylation-based subgroup information available in 112 samples [EPN_PFA (n = 72) and EPN_PFB tumors (n = 40)]. H3K27me3 staining was globally reduced in EPN_PFA tumors and immunohistochemistry showed 99
LB  - PUB:(DE-HGF)16
C6  - pmid:28733933
C2  - pmc:PMC5647236
DO  - DOI:10.1007/s00401-017-1752-4
UR  - https://inrepo02.dkfz.de/record/130893
ER  -