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@ARTICLE{Sahl:130903,
      author       = {S. J. Sahl and S. Hell$^*$ and S. Jakobs},
      title        = {{F}luorescence nanoscopy in cell biology.},
      journal      = {Nature reviews / Molecular cell biology},
      volume       = {18},
      number       = {11},
      issn         = {1471-0080},
      address      = {London},
      publisher    = {Macmillan},
      reportid     = {DKFZ-2017-05979},
      pages        = {685 - 701},
      year         = {2017},
      abstract     = {Fluorescence nanoscopy uniquely combines minimally invasive
                      optical access to the internal nanoscale structure and
                      dynamics of cells and tissues with molecular detection
                      specificity. While the basic physical principles of
                      super-resolution imaging were discovered in the 1990s, with
                      initial experimental demonstrations following in 2000, the
                      broad application of super-resolution imaging to address
                      cell-biological questions has only more recently emerged.
                      Nanoscopy approaches have begun to facilitate discoveries in
                      cell biology and to add new knowledge. One current direction
                      for method improvement is the ambition to quantitatively
                      account for each molecule under investigation and assess
                      true molecular colocalization patterns via multi-colour
                      analyses. In pursuing this goal, the labelling of individual
                      molecules to enable their visualization has emerged as a
                      central challenge. Extending nanoscale imaging into (sliced)
                      tissue and whole-animal contexts is a further goal. In this
                      Review we describe the successes to date and discuss current
                      obstacles and possibilities for further development.},
      subtyp        = {Review Article},
      cin          = {E190},
      ddc          = {570},
      cid          = {I:(DE-He78)E190-20160331},
      pnm          = {315 - Imaging and radiooncology (POF3-315)},
      pid          = {G:(DE-HGF)POF3-315},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:28875992},
      doi          = {10.1038/nrm.2017.71},
      url          = {https://inrepo02.dkfz.de/record/130903},
}