TY  - JOUR
AU  - Sawall, Stefan
AU  - Franke, Danielle
AU  - Kirchherr, Anne
AU  - Beckendorf, Jan
AU  - Kuntz, Jan
AU  - Maier, Joscha
AU  - Kraupner, Alexander
AU  - Backs, Johannes
AU  - Briel, Andreas
AU  - Kachelriess, Marc
TI  - In Vivo Quantification of Myocardial Infarction in Mice Using Micro-CT and a Novel Blood Pool Agent.
JO  - Contrast media & molecular imaging
VL  - 2017
SN  - 1555-4317
CY  - Hoboken, NJ
PB  - Wiley
M1  - DKFZ-2017-05981
SP  - 2617047
PY  - 2017
N1  - 2017 Oct 16;2017:2617047
AB  - We herein developed a micro-CT method using the innovative contrast agent ExiTron™ MyoC 8000 to longitudinally monitor cardiac processes in vivo in small animals. Experiments were performed on healthy mice and mice with myocardial infarction inflicted by ligation of the left anterior descending artery. Time-dependent signal enhancement in different tissues of healthy mice was measured and various contrast agent doses were investigated so as to determine the minimum required dose for imaging of the myocardium. Due to its ability to be taken up by healthy myocardium but not by infarct tissue, ExiTron MyoC 8000 enables detection of myocardial infarction even at a very low dose. The signal enhancement in the myocardium of infarcted mice after contrast agent injection was exploited for quantification of infarct size. The values of infarct size obtained from the imaging method were compared with those obtained from histology and showed a significant correlation (R(2) = 0.98). Thus, the developed micro-CT method allows for monitoring of a variety of processes such as cardiac remodeling in longitudinal studies.
LB  - PUB:(DE-HGF)16
C6  - pmid:29114173
C2  - pmc:PMC5662822
DO  - DOI:10.1155/2017/2617047
UR  - https://inrepo02.dkfz.de/record/130905
ER  -