%0 Journal Article
%A Michel, Chloe
%A Miller, Corey N
%A Küchler, Rita
%A Brors, Benedikt
%A Anderson, Mark S
%A Kyewski, Bruno
%A Pinto, Sheena
%T Revisiting the Road Map of Medullary Thymic Epithelial Cell Differentiation.
%J The journal of immunology
%V 199
%N 10
%@ 1550-6606
%C Bethesda, Md.
%I Soc.
%M DKFZ-2017-06033
%P 3488 - 3503
%D 2017
%X The basic two-step terminal differentiation model of the medullary thymic epithelial cell (mTEC) lineage from immature MHC class II (MHCII)(lo) to mature MHCII(hi) mTECs has recently been extended to include a third stage, namely the post-Aire MHCII(lo) subset as identified by lineage-tracing models. However, a suitable surface marker distinguishing the phenotypically overlapping pre- from the post-Aire MHCII(lo) stage has been lacking. In this study, we introduce the lectin Tetragonolobus purpureas agglutinin (TPA) as a novel cell surface marker that allows for such delineation. Based on our data, we derived the following sequence of mTEC differentiation: TPA(lo)MHCII(lo) → TPA(lo)MHCII(hi) → TPA(hi)MHCII(hi) → TPA(hi)MHCII(lo) Surprisingly, in the steady-state postnatal thymus TPA(lo)MHCII(lo) pre-Aire rather than terminally differentiated post-Aire TPA(hi)MHCII(lo) mTECs were marked for apoptosis at an exceptionally high rate of ∼70
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:28993517
%R 10.4049/jimmunol.1700203
%U https://inrepo02.dkfz.de/record/130957