TY  - JOUR
AU  - Michel, Chloe
AU  - Miller, Corey N
AU  - Küchler, Rita
AU  - Brors, Benedikt
AU  - Anderson, Mark S
AU  - Kyewski, Bruno
AU  - Pinto, Sheena
TI  - Revisiting the Road Map of Medullary Thymic Epithelial Cell Differentiation.
JO  - The journal of immunology
VL  - 199
IS  - 10
SN  - 1550-6606
CY  - Bethesda, Md.
PB  - Soc.
M1  - DKFZ-2017-06033
SP  - 3488 - 3503
PY  - 2017
AB  - The basic two-step terminal differentiation model of the medullary thymic epithelial cell (mTEC) lineage from immature MHC class II (MHCII)(lo) to mature MHCII(hi) mTECs has recently been extended to include a third stage, namely the post-Aire MHCII(lo) subset as identified by lineage-tracing models. However, a suitable surface marker distinguishing the phenotypically overlapping pre- from the post-Aire MHCII(lo) stage has been lacking. In this study, we introduce the lectin Tetragonolobus purpureas agglutinin (TPA) as a novel cell surface marker that allows for such delineation. Based on our data, we derived the following sequence of mTEC differentiation: TPA(lo)MHCII(lo) → TPA(lo)MHCII(hi) → TPA(hi)MHCII(hi) → TPA(hi)MHCII(lo) Surprisingly, in the steady-state postnatal thymus TPA(lo)MHCII(lo) pre-Aire rather than terminally differentiated post-Aire TPA(hi)MHCII(lo) mTECs were marked for apoptosis at an exceptionally high rate of ∼70
LB  - PUB:(DE-HGF)16
C6  - pmid:28993517
DO  - DOI:10.4049/jimmunol.1700203
UR  - https://inrepo02.dkfz.de/record/130957
ER  -