% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Peng:131644,
      author       = {B. Peng and S. T. Weintraub and C. Coman and S. Ponnaiyan
                      and R. Sharma$^*$ and B. Tews$^*$ and D. Winter and R.
                      Ahrends},
      title        = {{A} {C}omprehensive {H}igh-{R}esolution {T}argeted
                      {W}orkflow for the {D}eep {P}rofiling of {S}phingolipids.},
      journal      = {Analytical chemistry},
      volume       = {89},
      number       = {22},
      issn         = {1520-6882},
      address      = {Columbus, Ohio},
      publisher    = {American Chemical Society},
      reportid     = {DKFZ-2017-06276},
      pages        = {12480 - 12487},
      year         = {2017},
      abstract     = {Sphingolipids make up a highly diverse group of
                      biomolecules that not only are membrane components but also
                      are involved in various cellular functions such as signaling
                      and protein sorting. To obtain a quantitative view of the
                      sphingolipidome, sensitive, accurate, and comprehensive
                      methods are needed. Here, we present a targeted
                      reversed-phase liquid chromatography-high-resolution mass
                      spectrometry-based workflow that significantly increases the
                      accuracy of measured sphingolipids by resolving nearly
                      isobaric and isobaric species; this is accomplished by a use
                      of (i) an optimized extraction procedure, (ii) a segmented
                      gradient, and (iii) parallel reaction monitoring of a
                      sphingolipid specific fragmentation pattern. The workflow
                      was benchmarked against an accepted sphingolipid model
                      system, the RAW 264.7 cell line, and 61 sphingolipids were
                      quantified over a dynamic range of 7 orders of magnitude,
                      with detection limits in the low femtomole per milligram of
                      protein level, making this workflow an extremely versatile
                      tool for high-throughput sphingolipidomics.},
      cin          = {V077},
      ddc          = {540},
      cid          = {I:(DE-He78)V077-20160331},
      pnm          = {319H - Addenda (POF3-319H)},
      pid          = {G:(DE-HGF)POF3-319H},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29039908},
      doi          = {10.1021/acs.analchem.7b03576},
      url          = {https://inrepo02.dkfz.de/record/131644},
}