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@ARTICLE{Feng:131720,
author = {W. Feng$^*$ and H.-K. Liu$^*$ and D. Kawauchi$^*$},
title = {{CRISPR}-engineered genome editing for the next generation
neurological disease modeling.},
journal = {Progress in neuro-psychopharmacology $\&$ biological
psychiatry},
volume = {81},
issn = {0278-5846},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {DKFZ-2018-00026},
pages = {459 - 467},
year = {2018},
note = {DKFZ-ZMBH-Allianz},
abstract = {Neurological disorders often occur because of failure of
proper brain development and/or appropriate maintenance of
neuronal circuits. In order to understand roles of causative
factors (e.g. genes, cell types) in disease development,
generation of solid animal models has been one of
straight-forward approaches. Recent next generation
sequencing studies on human patient-derived clinical samples
have identified various types of recurrent mutations in
individual neurological diseases. While these discoveries
have prompted us to evaluate impact of mutated genes on
these neurological diseases, a feasible but flexible genome
editing tool had remained to be developed. An advance of
genome editing technology using the clustered regularly
interspaced short palindromic repeats (CRISPR) with the
CRISPR-associated protein (Cas) offers us a tremendous
potential to create a variety of mutations in the cell,
leading to 'next generation' disease models carrying
disease-associated mutations. We will here review recent
progress of CRISPR-based brain disease modeling studies and
discuss future requirement to tackle current difficulties in
usage of these technologies.},
subtyp = {Review Article},
cin = {A240 / B062},
ddc = {610},
cid = {I:(DE-He78)A240-20160331 / I:(DE-He78)B062-20160331},
pnm = {312 - Functional and structural genomics (POF3-312)},
pid = {G:(DE-HGF)POF3-312},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28536069},
doi = {10.1016/j.pnpbp.2017.05.019},
url = {https://inrepo02.dkfz.de/record/131720},
}