TY  - JOUR
AU  - Martins, Leila
AU  - Bung, Raffaela
AU  - Koch, Stefan
AU  - Richter, Karsten
AU  - Schwarzmüller, Laura
AU  - Terhardt, Dorothee
AU  - Kurtulmus, Bahtiyar
AU  - Niehrs, Christof
AU  - Rouhi, Arefeh
AU  - Lohmann, Ingrid
AU  - Pereira, Gislene
AU  - Fröhling, Stefan
AU  - Glimm, Hanno
AU  - Scholl, Claudia
TI  - Stk33 is required for spermatid differentiation and male fertility in mice.
JO  - Developmental biology
VL  - 433
IS  - 1
SN  - 0012-1606
CY  - Amsterdam [u.a.]
PB  - Elsevier
M1  - DKFZ-2018-00036
SP  - 84 - 93
PY  - 2018
AB  - Spermiogenesis is the final phase during sperm cell development in which round spermatids undergo dramatic morphological changes to generate spermatozoa. Here we report that the serine/threonine kinase Stk33 is essential for the differentiation of round spermatids into functional sperm cells and male fertility. Constitutive Stk33 deletion in mice results in severely malformed and immotile spermatozoa that are particularly characterized by disordered structural tail elements. Stk33 expression first appears in primary spermatocytes, and targeted deletion of Stk33 in these cells recapitulates the defects observed in constitutive knockout mice, confirming a germ cell-intrinsic function. Stk33 protein resides in the cytoplasm and partially co-localizes with the caudal end of the manchette, a transient structure that guides tail elongation, in elongating spermatids, and loss of Stk33 leads to the appearance of a tight, straight and elongated manchette. Together, these results identify Stk33 as an essential regulator of spermatid differentiation and male fertility.
KW  - Protein-Serine-Threonine Kinases (NLM Chemicals)
KW  - Stk33 protein, mouse (NLM Chemicals)
LB  - PUB:(DE-HGF)16
C6  - pmid:29155043
DO  - DOI:10.1016/j.ydbio.2017.11.007
UR  - https://inrepo02.dkfz.de/record/131730
ER  -