TY  - JOUR
AU  - Rabe, Jan-Hinrich
AU  - A Sammour, Denis
AU  - Schulz, Sandra
AU  - Munteanu, Bogdan
AU  - Ott, Martina
AU  - Ochs, Katharina
AU  - Hohenberger, Peter
AU  - Marx, Alexander
AU  - Platten, Michael
AU  - Opitz, Christiane A
AU  - Ory, Daniel S
AU  - Hopf, Carsten
TI  - Fourier Transform Infrared Microscopy Enables Guidance of Automated Mass Spectrometry Imaging to Predefined Tissue Morphologies.
JO  - Scientific reports
VL  - 8
IS  - 1
SN  - 2045-2322
CY  - London
PB  - Nature Publishing Group
M1  - DKFZ-2018-00099
SP  - 313
PY  - 2018
AB  - Multimodal imaging combines complementary platforms for spatially resolved tissue analysis that are poised for application in life science and personalized medicine. Unlike established clinical in vivo multimodality imaging, automated workflows for in-depth multimodal molecular ex vivo tissue analysis that combine the speed and ease of spectroscopic imaging with molecular details provided by mass spectrometry imaging (MSI) are lagging behind. Here, we present an integrated approach that utilizes non-destructive Fourier transform infrared (FTIR) microscopy and matrix assisted laser desorption/ionization (MALDI) MSI for analysing single-slide tissue specimen. We show that FTIR microscopy can automatically guide high-resolution MSI data acquisition and interpretation without requiring prior histopathological tissue annotation, thus circumventing potential human-annotation-bias while achieving >90
LB  - PUB:(DE-HGF)16
C6  - pmid:29321555
C2  - pmc:PMC5762902
DO  - DOI:10.1038/s41598-017-18477-6
UR  - https://inrepo02.dkfz.de/record/131802
ER  -