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000132592 037__ $$aDKFZ-2018-00256
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000132592 1001_ $$aRajagopala, Seesandra V$$b0
000132592 245__ $$aThe binary protein-protein interaction landscape of Escherichia coli.
000132592 260__ $$aNew York, NY$$bNature America$$c2014
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000132592 520__ $$aEfforts to map the Escherichia coli interactome have identified several hundred macromolecular complexes, but direct binary protein-protein interactions (PPIs) have not been surveyed on a large scale. Here we performed yeast two-hybrid screens of 3,305 baits against 3,606 preys (∼70% of the E. coli proteome) in duplicate to generate a map of 2,234 interactions, which approximately doubles the number of known binary PPIs in E. coli. Integration of binary PPI and genetic-interaction data revealed functional dependencies among components involved in cellular processes, including envelope integrity, flagellum assembly and protein quality control. Many of the binary interactions that we could map in multiprotein complexes were informative regarding internal topology of complexes and indicated that interactions in complexes are substantially more conserved than those interactions connecting different complexes. This resource will be useful for inferring bacterial gene function and provides a draft reference of the basic physical wiring network of this evolutionarily important model microbe.
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000132592 650_7 $$2NLM Chemicals$$aEscherichia coli Proteins
000132592 7001_ $$aSikorski, Patricia$$b1
000132592 7001_ $$aKumar, Ashwani$$b2
000132592 7001_ $$aMosca, Roberto$$b3
000132592 7001_ $$aVlasblom, James$$b4
000132592 7001_ $$aArnold, Roland$$b5
000132592 7001_ $$aFranca-Koh, Jonathan$$b6
000132592 7001_ $$aPakala, Suman B$$b7
000132592 7001_ $$aPhanse, Sadhna$$b8
000132592 7001_ $$aCeol, Arnaud$$b9
000132592 7001_ $$0P:(DE-HGF)0$$aHäuser, Roman$$b10
000132592 7001_ $$0P:(DE-HGF)0$$aSiszler, Gabriella$$b11
000132592 7001_ $$aWuchty, Stefan$$b12
000132592 7001_ $$aEmili, Andrew$$b13
000132592 7001_ $$aBabu, Mohan$$b14
000132592 7001_ $$aAloy, Patrick$$b15
000132592 7001_ $$aPieper, Rembert$$b16
000132592 7001_ $$aUetz, Peter$$b17
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