guest ::
| Home > Publications database > The binary protein-protein interaction landscape of Escherichia coli. > print |
| Home > Publications database > The binary protein-protein interaction landscape of Escherichia coli. > print |
| 001 | 132592 | ||
| 005 | 20240228135110.0 | ||
| 024 | 7 | _ | |a 10.1038/nbt.2831 |2 doi |
| 024 | 7 | _ | |a pmid:24561554 |2 pmid |
| 024 | 7 | _ | |a pmc:PMC4123855 |2 pmc |
| 024 | 7 | _ | |a 0733-222X |2 ISSN |
| 024 | 7 | _ | |a 1087-0156 |2 ISSN |
| 024 | 7 | _ | |a 1546-1696 |2 ISSN |
| 024 | 7 | _ | |a altmetric:2139950 |2 altmetric |
| 037 | _ | _ | |a DKFZ-2018-00256 |
| 041 | _ | _ | |a eng |
| 082 | _ | _ | |a 570 |
| 100 | 1 | _ | |a Rajagopala, Seesandra V |b 0 |
| 245 | _ | _ | |a The binary protein-protein interaction landscape of Escherichia coli. |
| 260 | _ | _ | |a New York, NY |c 2014 |b Nature America |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1521633796_923 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Efforts to map the Escherichia coli interactome have identified several hundred macromolecular complexes, but direct binary protein-protein interactions (PPIs) have not been surveyed on a large scale. Here we performed yeast two-hybrid screens of 3,305 baits against 3,606 preys (∼70% of the E. coli proteome) in duplicate to generate a map of 2,234 interactions, which approximately doubles the number of known binary PPIs in E. coli. Integration of binary PPI and genetic-interaction data revealed functional dependencies among components involved in cellular processes, including envelope integrity, flagellum assembly and protein quality control. Many of the binary interactions that we could map in multiprotein complexes were informative regarding internal topology of complexes and indicated that interactions in complexes are substantially more conserved than those interactions connecting different complexes. This resource will be useful for inferring bacterial gene function and provides a draft reference of the basic physical wiring network of this evolutionarily important model microbe. |
| 536 | _ | _ | |a 312 - Functional and structural genomics (POF3-312) |0 G:(DE-HGF)POF3-312 |c POF3-312 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef, PubMed, |
| 650 | _ | 7 | |a Escherichia coli Proteins |2 NLM Chemicals |
| 700 | 1 | _ | |a Sikorski, Patricia |b 1 |
| 700 | 1 | _ | |a Kumar, Ashwani |b 2 |
| 700 | 1 | _ | |a Mosca, Roberto |b 3 |
| 700 | 1 | _ | |a Vlasblom, James |b 4 |
| 700 | 1 | _ | |a Arnold, Roland |b 5 |
| 700 | 1 | _ | |a Franca-Koh, Jonathan |b 6 |
| 700 | 1 | _ | |a Pakala, Suman B |b 7 |
| 700 | 1 | _ | |a Phanse, Sadhna |b 8 |
| 700 | 1 | _ | |a Ceol, Arnaud |b 9 |
| 700 | 1 | _ | |a Häuser, Roman |0 P:(DE-HGF)0 |b 10 |
| 700 | 1 | _ | |a Siszler, Gabriella |0 P:(DE-HGF)0 |b 11 |
| 700 | 1 | _ | |a Wuchty, Stefan |b 12 |
| 700 | 1 | _ | |a Emili, Andrew |b 13 |
| 700 | 1 | _ | |a Babu, Mohan |b 14 |
| 700 | 1 | _ | |a Aloy, Patrick |b 15 |
| 700 | 1 | _ | |a Pieper, Rembert |b 16 |
| 700 | 1 | _ | |a Uetz, Peter |b 17 |
| 773 | _ | _ | |a 10.1038/nbt.2831 |g Vol. 32, no. 3, p. 285 - 290 |0 PERI:(DE-600)1494943-x |n 3 |p 285 - 290 |t Nature biotechnology |v 32 |y 2014 |x 1546-1696 |
| 909 | C | O | |o oai:inrepo02.dkfz.de:132592 |p VDB |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 10 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Deutsches Krebsforschungszentrum |0 I:(DE-588b)2036810-0 |k DKFZ |b 11 |6 P:(DE-HGF)0 |
| 913 | 1 | _ | |a DE-HGF |l Krebsforschung |1 G:(DE-HGF)POF3-310 |0 G:(DE-HGF)POF3-312 |2 G:(DE-HGF)POF3-300 |v Functional and structural genomics |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |b Gesundheit |
| 914 | 1 | _ | |y 2014 |
| 915 | _ | _ | |a Nationallizenz |0 StatID:(DE-HGF)0420 |2 StatID |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b NAT BIOTECHNOL : 2015 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0600 |2 StatID |b Ebsco Academic Search |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b ASC |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1060 |2 StatID |b Current Contents - Agriculture, Biology and Environmental Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |
| 915 | _ | _ | |a IF >= 40 |0 StatID:(DE-HGF)9940 |2 StatID |b NAT BIOTECHNOL : 2015 |
| 920 | 1 | _ | |0 I:(DE-He78)W150-20160331 |k W150 |l Proteininteraktion |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-He78)W150-20160331 |
| 980 | _ | _ | |a UNRESTRICTED |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|