TY  - JOUR
AU  - Gómez, Soledad
AU  - Garrido-Garcia, Alícia
AU  - Garcia-Gerique, Laura
AU  - Lemos, Isadora
AU  - Suñol, Mariona
AU  - de Torres, Carmen
AU  - Kulis, Marta
AU  - Pérez-Jaume, Sara
AU  - Carcaboso, Ángel M
AU  - Luu, Betty
AU  - Kieran, Mark W
AU  - Jabado, Nada
AU  - Kozlenkov, Alexey
AU  - Dracheva, Stella
AU  - Ramaswamy, Vijay
AU  - Hovestadt, Volker
AU  - Johann, Pascal
AU  - Jones, David
AU  - Pfister, Stefan
AU  - Morales La Madrid, Andrés
AU  - Cruz, Ofelia
AU  - Taylor, Michael D
AU  - Martin-Subero, Jose-Ignacio
AU  - Mora, Jaume
AU  - Lavarino, Cinzia
TI  - A Novel Method for Rapid Molecular Subgrouping of Medulloblastoma.
JO  - Clinical cancer research
VL  - 24
IS  - 6
SN  - 1557-3265
CY  - Philadelphia, Pa. [u.a.]
PB  - AACR
M1  - DKFZ-2018-00393
SP  - 1355 - 1363
PY  - 2018
AB  - Purpose: The classification of medulloblastoma into WNT, SHH, group 3, and group 4 subgroups has become of critical importance for patient risk stratification and subgroup-tailored clinical trials. Here, we aimed to develop a simplified, clinically applicable classification approach that can be implemented in the majority of centers treating patients with medulloblastoma.Experimental Design: We analyzed 1,577 samples comprising previously published DNA methylation microarray data (913 medulloblastomas, 457 non-medulloblastoma tumors, 85 normal tissues), and 122 frozen and formalin-fixed paraffin-embedded medulloblastoma samples. Biomarkers were identified applying stringent selection filters and Linear Discriminant Analysis (LDA) method, and validated using DNA methylation microarray data, bisulfite pyrosequencing, and direct-bisulfite sequencing.Results: Using a LDA-based approach, we developed and validated a prediction method (EpiWNT-SHH classifier) based on six epigenetic biomarkers that allowed for rapid classification of medulloblastoma into the clinically relevant subgroups WNT, SHH, and non-WNT/non-SHH with excellent concordance (>99
LB  - PUB:(DE-HGF)16
C6  - pmid:29351917
DO  - DOI:10.1158/1078-0432.CCR-17-2243
UR  - https://inrepo02.dkfz.de/record/132739
ER  -