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000132781 0247_ $$2doi$$a10.1089/ten.tea.2017.0107
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000132781 0247_ $$2ISSN$$a1937-3341
000132781 0247_ $$2ISSN$$a1937-335X
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000132781 037__ $$aDKFZ-2018-00425
000132781 041__ $$aeng
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000132781 1001_ $$aAkhyari, Payam$$b0
000132781 245__ $$aCharacterization of the Epicardial Adipose Tissue in Decellularized Human-Scaled Whole Hearts: Implications for the Whole-Heart Tissue Engineering.
000132781 260__ $$aLarchmont, NY$$bLiebert$$c2018
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000132781 520__ $$aWhole-organ engineering is an innovative field of regenerative medicine with growing translational perspectives. Recent reports suggest the feasibility of decellularization and repopulation of entire human size hearts. However, little is known about the susceptibility of epicardial adipose tissue (EAT) to decellularization. In this study, human size hearts of ovine donors were subjected to perfusion-based decellularization using detergent solutions. Upon basic histological evaluation and total DNA measurement myocardial regions prove largely decellularized while EAT demonstrated cellular remnants, further confirmed by transmission electron microscopy. Western blot analysis showed a significant reduction in lipid-associated and cardiac proteins. However, gas chromatography revealed unchanged proportional composition of fatty acids in EAT of decellularized whole hearts. Finally, cell culture medium conditioned with EAT from decellularized whole hearts had a significant deleterious effect on cardiac fibroblasts. These data suggest that perfusion decellularization of human size whole hearts provides inconsistent efficacy regarding donor material removal from myocardial regions as opposed to EAT.
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000132781 7001_ $$aOberle, Fabian$$b1
000132781 7001_ $$aHülsmann, Jörn$$b2
000132781 7001_ $$0P:(DE-He78)c63edb4f71da4cb5b1316391da0f209c$$aHeid, Hans$$b3$$udkfz
000132781 7001_ $$aLehr, Stefan$$b4
000132781 7001_ $$aBarbian, Andreas$$b5
000132781 7001_ $$aNakanishi, Sentaro$$b6
000132781 7001_ $$aAubin, Hug$$b7
000132781 7001_ $$aJenke, Alexander$$b8
000132781 7001_ $$aLichtenberg, Artur$$b9
000132781 773__ $$0PERI:(DE-600)2401807-7$$a10.1089/ten.tea.2017.0107$$gVol. 24, no. 7-8, p. 682 - 693$$n7-8$$p682 - 693$$tTissue engineering / A$$v24$$x1937-335X$$y2018
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000132781 9141_ $$y2018
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