TY  - JOUR
AU  - Akhyari, Payam
AU  - Oberle, Fabian
AU  - Hülsmann, Jörn
AU  - Heid, Hans
AU  - Lehr, Stefan
AU  - Barbian, Andreas
AU  - Nakanishi, Sentaro
AU  - Aubin, Hug
AU  - Jenke, Alexander
AU  - Lichtenberg, Artur
TI  - Characterization of the Epicardial Adipose Tissue in Decellularized Human-Scaled Whole Hearts: Implications for the Whole-Heart Tissue Engineering.
JO  - Tissue engineering / A
VL  - 24
IS  - 7-8
SN  - 1937-335X
CY  - Larchmont, NY
PB  - Liebert
M1  - DKFZ-2018-00425
SP  - 682 - 693
PY  - 2018
AB  - Whole-organ engineering is an innovative field of regenerative medicine with growing translational perspectives. Recent reports suggest the feasibility of decellularization and repopulation of entire human size hearts. However, little is known about the susceptibility of epicardial adipose tissue (EAT) to decellularization. In this study, human size hearts of ovine donors were subjected to perfusion-based decellularization using detergent solutions. Upon basic histological evaluation and total DNA measurement myocardial regions prove largely decellularized while EAT demonstrated cellular remnants, further confirmed by transmission electron microscopy. Western blot analysis showed a significant reduction in lipid-associated and cardiac proteins. However, gas chromatography revealed unchanged proportional composition of fatty acids in EAT of decellularized whole hearts. Finally, cell culture medium conditioned with EAT from decellularized whole hearts had a significant deleterious effect on cardiac fibroblasts. These data suggest that perfusion decellularization of human size whole hearts provides inconsistent efficacy regarding donor material removal from myocardial regions as opposed to EAT.
LB  - PUB:(DE-HGF)16
C6  - pmid:28895502
DO  - DOI:10.1089/ten.tea.2017.0107
UR  - https://inrepo02.dkfz.de/record/132781
ER  -