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@ARTICLE{Akhyari:132781,
      author       = {P. Akhyari and F. Oberle and J. Hülsmann and H. Heid$^*$
                      and S. Lehr and A. Barbian and S. Nakanishi and H. Aubin and
                      A. Jenke and A. Lichtenberg},
      title        = {{C}haracterization of the {E}picardial {A}dipose {T}issue
                      in {D}ecellularized {H}uman-{S}caled {W}hole {H}earts:
                      {I}mplications for the {W}hole-{H}eart {T}issue
                      {E}ngineering.},
      journal      = {Tissue engineering / A},
      volume       = {24},
      number       = {7-8},
      issn         = {1937-335X},
      address      = {Larchmont, NY},
      publisher    = {Liebert},
      reportid     = {DKFZ-2018-00425},
      pages        = {682 - 693},
      year         = {2018},
      abstract     = {Whole-organ engineering is an innovative field of
                      regenerative medicine with growing translational
                      perspectives. Recent reports suggest the feasibility of
                      decellularization and repopulation of entire human size
                      hearts. However, little is known about the susceptibility of
                      epicardial adipose tissue (EAT) to decellularization. In
                      this study, human size hearts of ovine donors were subjected
                      to perfusion-based decellularization using detergent
                      solutions. Upon basic histological evaluation and total DNA
                      measurement myocardial regions prove largely decellularized
                      while EAT demonstrated cellular remnants, further confirmed
                      by transmission electron microscopy. Western blot analysis
                      showed a significant reduction in lipid-associated and
                      cardiac proteins. However, gas chromatography revealed
                      unchanged proportional composition of fatty acids in EAT of
                      decellularized whole hearts. Finally, cell culture medium
                      conditioned with EAT from decellularized whole hearts had a
                      significant deleterious effect on cardiac fibroblasts. These
                      data suggest that perfusion decellularization of human size
                      whole hearts provides inconsistent efficacy regarding donor
                      material removal from myocardial regions as opposed to EAT.},
      cin          = {A991},
      ddc          = {570},
      cid          = {I:(DE-He78)A991-20160331},
      pnm          = {311 - Signalling pathways, cell and tumor biology
                      (POF3-311)},
      pid          = {G:(DE-HGF)POF3-311},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:28895502},
      doi          = {10.1089/ten.tea.2017.0107},
      url          = {https://inrepo02.dkfz.de/record/132781},
}