%0 Journal Article
%A Weigand, Tim
%A Singler, Benjamin
%A Fleming, Thomas
%A Nawroth, Peter
%A Klika, Karel
%A Thiel, Christian
%A Baelde, Hans
%A Garbade, Sven F
%A Wagner, Andreas H
%A Hecker, Markus
%A Yard, Benito A
%A Amberger, Albert
%A Zschocke, Johannes
%A Schmitt, Claus P
%A Peters, Verena
%T Carnosine Catalyzes the Formation of the Oligo/Polymeric Products of Methylglyoxal.
%J Cellular physiology and biochemistry
%V 46
%N 2
%@ 1421-9778
%C Basel
%I Karger
%M DKFZ-2018-00588
%P 713 - 726
%D 2018
%X Reactive dicarbonyl compounds, such as methylglyoxal (MG), contribute to diabetic complications. MG-scavenging capacities of carnosine and anserine, which have been shown to mitigate diabetic nephropathy, were evaluated in vitro and in vivo.MG-induced cell toxicity was characterized by MTT and MG-H1-formation, scavenging abilities by Western Blot and NMR spectroscopies, cellular carnosine transport by qPCR and microplate luminescence and carnosine concentration by HPLC.In vitro, carnosine and anserine dose-dependently reduced N-carboxyethyl lysine (CEL) and advanced glycation end products (AGEs) formation. NMR studies revealed the formation of oligo/polymeric products of MG catalyzed by carnosine or anserine. MG toxicity (0.3-1 mM) was dose-dependent for podocytes, tubular and mesangial cells whereas low MG levels (0.2 mM) resulted in increased cell viability in podocytes (143±13
%F PUB:(DE-HGF)16
%9 Journal Article
%$ pmid:29621776
%R 10.1159/000488727
%U https://inrepo02.dkfz.de/record/132949