TY  - JOUR
AU  - Weigand, Tim
AU  - Singler, Benjamin
AU  - Fleming, Thomas
AU  - Nawroth, Peter
AU  - Klika, Karel
AU  - Thiel, Christian
AU  - Baelde, Hans
AU  - Garbade, Sven F
AU  - Wagner, Andreas H
AU  - Hecker, Markus
AU  - Yard, Benito A
AU  - Amberger, Albert
AU  - Zschocke, Johannes
AU  - Schmitt, Claus P
AU  - Peters, Verena
TI  - Carnosine Catalyzes the Formation of the Oligo/Polymeric Products of Methylglyoxal.
JO  - Cellular physiology and biochemistry
VL  - 46
IS  - 2
SN  - 1421-9778
CY  - Basel
PB  - Karger
M1  - DKFZ-2018-00588
SP  - 713 - 726
PY  - 2018
AB  - Reactive dicarbonyl compounds, such as methylglyoxal (MG), contribute to diabetic complications. MG-scavenging capacities of carnosine and anserine, which have been shown to mitigate diabetic nephropathy, were evaluated in vitro and in vivo.MG-induced cell toxicity was characterized by MTT and MG-H1-formation, scavenging abilities by Western Blot and NMR spectroscopies, cellular carnosine transport by qPCR and microplate luminescence and carnosine concentration by HPLC.In vitro, carnosine and anserine dose-dependently reduced N-carboxyethyl lysine (CEL) and advanced glycation end products (AGEs) formation. NMR studies revealed the formation of oligo/polymeric products of MG catalyzed by carnosine or anserine. MG toxicity (0.3-1 mM) was dose-dependent for podocytes, tubular and mesangial cells whereas low MG levels (0.2 mM) resulted in increased cell viability in podocytes (143±13
LB  - PUB:(DE-HGF)16
C6  - pmid:29621776
DO  - DOI:10.1159/000488727
UR  - https://inrepo02.dkfz.de/record/132949
ER  -