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@ARTICLE{Michel:134835,
      author       = {C. Michel$^*$ and R. Küchler$^*$ and I. Martin$^*$ and B.
                      Kyewski$^*$ and S. Pinto$^*$},
      title        = {{A}n efficient protocol for in vivo labeling of
                      proliferating epithelial cells.},
      journal      = {Journal of immunological methods},
      volume       = {457},
      issn         = {0022-1759},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {DKFZ-2018-00625},
      pages        = {82 - 86},
      year         = {2018},
      abstract     = {The study of organogenesis, tissue-homeostasis and
                      regeneration requires the precise assessment of in vivo cell
                      proliferation. To this end a host of methods have been
                      developed to detect and quantify DNA synthesis in
                      proliferating cells. These include cell labeling with
                      various nucleotide analogues and fluorescence reporter-based
                      animal models with each method presenting its idiosyncratic
                      shortcomings. Quantitative assessment of epithelial cell
                      turnover has been partly hampered due to their variable and
                      limited in vivo accessibility and the requirement for
                      harsher isolation procedures to procure single cells for
                      FACS analysis. Here, we report a reliable protocol to study
                      in vivo cell proliferation of epithelial cells in mice by
                      repeatedly injecting EdU intravenously for an extended
                      12-day period. EdU incorporation was quantitated ex vivo by
                      FACS after tissue dissociation in order to obtain single
                      epithelial cell suspensions. As a lead population, we
                      analyzed thymic epithelial cells (TECs), where we were able
                      to label compartmentalized TEC subsets to saturation without
                      apparent toxic effects on the thymus architecture or
                      stress-sensitive TEC lineage differentiation. The data is in
                      concordance with the prevailing model of medullary TEC
                      terminal differentiation that includes the post-Aire stage.
                      The same protocol was successfully applied to epithelial
                      cells of various other organs - skin, lymph node, kidney and
                      small intestine - tissues with widely varying frequencies
                      and rates of proliferating epithelial cells.},
      cin          = {D090 / A110},
      ddc          = {610},
      cid          = {I:(DE-He78)D090-20160331 / I:(DE-He78)A110-20160331},
      pnm          = {314 - Tumor immunology (POF3-314)},
      pid          = {G:(DE-HGF)POF3-314},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29605230},
      doi          = {10.1016/j.jim.2018.03.015},
      url          = {https://inrepo02.dkfz.de/record/134835},
}