TY  - JOUR
AU  - Pfaff, Elke
AU  - Kessler, Tobias
AU  - Balasubramanian, Gnana Prakash
AU  - Berberich, Anne
AU  - Schrimpf, Daniel
AU  - Wick, Antje
AU  - Debus, Jürgen
AU  - Unterberg, Andreas
AU  - Bendszus, Martin
AU  - Herold-Mende, Christel
AU  - Capper, David
AU  - Schenkel, Irini
AU  - Eisenmenger, Andreas
AU  - Dettmer, Susan
AU  - Brors, Benedikt
AU  - Platten, Michael
AU  - Pfister, Stefan
AU  - von Deimling, Andreas
AU  - Jones, David
AU  - Wick, Wolfgang
AU  - Sahm, Felix
TI  - Feasibility of real-time molecular profiling for patients with newly diagnosed glioblastoma without MGMT promoter hypermethylation-the NCT Neuro Master Match (N2M2) pilot study.
JO  - Neuro-Oncology
VL  - 20
IS  - 6
SN  - 1523-5866
CY  - Oxford
PB  - Oxford Univ. Press
M1  - DKFZ-2018-00653
SP  - 826 - 837
PY  - 2018
N1  - Pfaff E*, Kessler T*  (*first author") / David T. W. Jones,# Wolfgang Wick,# and Felix Sahm# last authors
AB  - O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation status is a predictive biomarker in glioblastoma patients. Glioblastoma without hypermethylated MGMT promoter is largely resistant to treatment with temozolomide. These patients are in particular need of new treatment approaches, which are offered by biomarker-driven clinical trials with targeted drugs based on molecular characterization of individual tumors.In preparation for an upcoming clinical study, a comprehensive molecular profiling approach was undertaken on tissues from 43 glioblastoma patients harboring an unmethylated MGMT promoter at diagnosis. The diagnostic pipeline covered various levels of molecular characteristics, including whole-exome sequencing, low-coverage whole-genome sequencing, RNA sequencing, as well as microarray-based gene expression profiling and DNA methylation arrays.Complex multilayer molecular diagnostics were feasible in this setting with a median turnaround time of 4-5 weeks from surgery to the molecular tumor board. In 35
LB  - PUB:(DE-HGF)16
C6  - pmid:29165638
C2  - pmc:PMC5961072
DO  - DOI:10.1093/neuonc/nox216
UR  - https://inrepo02.dkfz.de/record/134863
ER  -