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@ARTICLE{Culina:136022,
author = {S. Culina and A. I. Lalanne and G. Afonso and K.
Cerosaletti and S. Pinto$^*$ and G. Sebastiani and K.
Kuranda and L. Nigi and A. Eugster and T. Østerbye and A.
Maugein and J. E. McLaren and K. Ladell and E. Larger and
J.-P. Beressi and A. Lissina and V. Appay and H. W. Davidson
and S. Buus and D. A. Price and M. Kuhn and E. Bonifacio and
M. Battaglia and S. Caillat-Zucman and F. Dotta and R.
Scharfmann and B. Kyewski$^*$ and R. Mallone and J.-C. Carel
and N. Tubiana-Rufi and L. Martinerie and A. Poidvin and E.
JacqzAigrain and L. Corvez and V. Berruer and J.-F. Gautier
and B. Baz and N. Haddadi and F. Andreelli and C. Amouyal
and S. Jaqueminet and O. Bourron and E. Dasque and A.
Hartemann and A. Lemoine-Yazigi and D. Dubois-Laforgue and
F. Travert and M. Feron and P. Rolland and V. Vignali and M.
Marre and P. Chanson and C. Briet and P.-J. Guillausseau and
L. Ait-Bachir and C. Collet and F. Beziaud and V.
Desforges-Bullet and G. Petit-Aubert and S. Christin-Maitre
and B. Fève and C. Vatier and N. Bourcigaux and C.
Lautridou and N. Lahlou and P. Bakouboula and C. Elie and H.
Morel and J.-M. Treluyer and M.-C. Gagnerault and C.
Maillard and A. Jones},
collaboration = {I. S. Group},
title = {{I}slet-reactive {CD}8+ {T} cell frequencies in the
pancreas, but not in blood, distinguish type 1 diabetic
patients from healthy donors.},
journal = {Science immunology},
volume = {3},
number = {20},
issn = {2470-9468},
address = {Washington, DC},
publisher = {AAAS},
reportid = {DKFZ-2018-00722},
pages = {eaao4013 -},
year = {2018},
abstract = {The human leukocyte antigen-A2 (HLA-A2)-restricted zinc
transporter 8186-194 (ZnT8186-194) and other islet epitopes
elicit interferon-γ secretion by CD8+ T cells
preferentially in type 1 diabetes (T1D) patients compared
with controls. We show that clonal ZnT8186-194-reactive CD8+
T cells express private T cell receptors and display
equivalent functional properties in T1D and healthy
individuals. Ex vivo analyses further revealed that CD8+ T
cells reactive to ZnT8186-194 and other islet epitopes
circulate at similar frequencies and exhibit a predominantly
naïve phenotype in age-matched T1D and healthy donors.
Higher frequencies of ZnT8186-194-reactive CD8+ T cells with
a more antigen-experienced phenotype were detected in
children versus adults, irrespective of disease status.
Moreover, some ZnT8186-194-reactive CD8+ T cell clonotypes
were found to cross-recognize a Bacteroides stercoris
mimotope. Whereas ZnT8 was poorly expressed in thymic
medullary epithelial cells, variable thymic expression
levels of islet antigens did not modulate the peripheral
frequency of their cognate CD8+ T cells. In contrast,
ZnT8186-194-reactive cells were enriched in the pancreata of
T1D patients versus nondiabetic and type 2 diabetic
individuals. Thus, islet-reactive CD8+ T cells circulate in
most individuals but home to the pancreas preferentially in
T1D patients. We conclude that the activation of this common
islet-reactive T cell repertoire and progression to T1D
likely require defective peripheral immunoregulation and/or
a proinflammatory islet microenvironment.},
cin = {D090 ; D090},
ddc = {610},
cid = {I:(DE-He78)D090-20160331},
pnm = {314 - Tumor immunology (POF3-314)},
pid = {G:(DE-HGF)POF3-314},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29429978},
pmc = {pmc:PMC5874133},
doi = {10.1126/sciimmunol.aao4013},
url = {https://inrepo02.dkfz.de/record/136022},
}