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@ARTICLE{Jabs:136031,
author = {M. Jabs$^*$ and A. J. Rose and L. H. Lehmann and J.
Taylor$^*$ and I. Moll$^*$ and T. Sijmonsma$^*$ and S. E.
Herberich$^*$ and S. W. Sauer and G. Poschet and G.
Federico$^*$ and C. Mogler and E.-M. Weis$^*$ and H.
Augustin$^*$ and M. Yan and N. Gretz and R. M. Schmid and R.
H. Adams and H.-J. Gröne and R. Hell and J. G. Okun and J.
Backs and P. P. Nawroth and S. Herzig and A. Fischer$^*$},
title = {{I}nhibition of {E}ndothelial {N}otch {S}ignaling {I}mpairs
{F}atty {A}cid {T}ransport and {L}eads to {M}etabolic and
{V}ascular {R}emodeling of the {A}dult {H}eart.},
journal = {Circulation},
volume = {137},
number = {24},
issn = {1524-4539},
address = {Philadelphia, Pa.},
publisher = {Lippincott, Williams $\&$ Wilkins},
reportid = {DKFZ-2018-00731},
pages = {2592 - 2608},
year = {2018},
abstract = {Nutrients are transported through endothelial cells before
being metabolized in muscle cells. However, little is known
about the regulation of endothelial transport processes.
Notch signaling is a critical regulator of metabolism and
angiogenesis during development. Here, we studied how
genetic and pharmacological manipulation of endothelial
Notch signaling in adult mice affects endothelial fatty acid
transport, cardiac angiogenesis, and heart
function.Endothelial-specific Notch inhibition was achieved
by conditional genetic inactivation of Rbp-jκ in adult mice
to analyze fatty acid metabolism and heart function.
Wild-type mice were treated with neutralizing antibodies
against the Notch ligand Delta-like 4. Fatty acid transport
was studied in cultured endothelial cells and transgenic
mice.Treatment of wild-type mice with Delta-like 4
neutralizing antibodies for 8 weeks impaired fractional
shortening and ejection fraction in the majority of mice.
Inhibition of Notch signaling specifically in the
endothelium of adult mice by genetic ablation of Rbp-jκ
caused heart hypertrophy and failure. Impaired heart
function was preceded by alterations in fatty acid
metabolism and an increase in cardiac blood vessel density.
Endothelial Notch signaling controlled the expression of
endothelial lipase, Angptl4, CD36, and Fabp4, which are all
needed for fatty acid transport across the vessel wall. In
endothelial-specific Rbp-jκ-mutant mice, lipase activity
and transendothelial transport of long-chain fatty acids to
muscle cells were impaired. In turn, lipids accumulated in
the plasma and liver. The attenuated supply of
cardiomyocytes with long-chain fatty acids was accompanied
by higher glucose uptake, increased concentration of
glycolysis intermediates, and mTOR-S6K signaling. Treatment
with the mTOR inhibitor rapamycin or displacing glucose as
cardiac substrate by feeding a ketogenic diet prolonged the
survival of endothelial-specific Rbp-jκ-deficient mice.This
study identifies Notch signaling as a novel regulator of
fatty acid transport across the endothelium and as an
essential repressor of angiogenesis in the adult heart. The
data imply that the endothelium controls cardiomyocyte
metabolism and function.},
cin = {A270 / G130 / A190},
ddc = {610},
cid = {I:(DE-He78)A270-20160331 / I:(DE-He78)G130-20160331 /
I:(DE-He78)A190-20160331},
pnm = {321 - Basic Concepts (POF3-321)},
pid = {G:(DE-HGF)POF3-321},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29353241},
doi = {10.1161/CIRCULATIONAHA.117.029733},
url = {https://inrepo02.dkfz.de/record/136031},
}