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@ARTICLE{Wirthschaft:136833,
author = {P. Wirthschaft$^*$ and J. Bode$^*$ and A. Simon$^*$ and E.
Hoffmann$^*$ and R. van Laack$^*$ and T. Krüwel$^*$ and F.
Dietrich$^*$ and D. Bucher$^*$ and A. Hahn and F. Sahm$^*$
and M. Breckwoldt$^*$ and F. T. Kurz and T. Hielscher$^*$
and B. Fischer$^*$ and N. Dross and C. Ruiz de Almodovar and
A. von Deimling$^*$ and C. Herold-Mende and C. Plass$^*$ and
S. Boulant$^*$ and B. P. O. Wiestler$^*$ and G.
Reifenberger$^*$ and P. Lichter$^*$ and W. Wick$^*$ and B.
Tews$^*$},
title = {{A} {PRDX}1-p38α heterodimer amplifies {MET}-driven
invasion of {IDH}-wildtype and {IDH}-mutant gliomas.},
journal = {International journal of cancer},
volume = {143},
number = {5},
issn = {0020-7136},
address = {Bognor Regis},
publisher = {Wiley-Liss},
reportid = {DKFZ-2018-01271},
pages = {1176 - 1187},
year = {2018},
abstract = {The Peroxiredoxin 1 (PRDX1) gene maps to chromosome arm 1p
and is hemizygously deleted and epigenetically silenced in
isocitrate dehydrogenase 1 or 2 (IDH)-mutant and
1p/19q-codeleted oligodendroglial tumors. In contrast,
IDH-wildtype astrocytic gliomas including glioblastomas
mostly lack epigenetic silencing and express PRDX1 protein.
In our study, we investigated how PRDX1 contributes to the
infiltrative growth of IDH-wildtype gliomas. Focusing on
p38α-dependent pathways, we analyzed clinical data from 133
patients of the NOA-04 trial cohort to look for differences
in the gene expression profiles of gliomas with wildtype or
mutant IDH. Biochemical interaction studies as well as in
vitro and ex vivo migration studies were used to establish a
biological role of PRDX1 in maintaining pathway activity.
Whole-brain high-resolution ultramicroscopy and survival
analyses of pre-clinical mouse models for IDH-wildtype
gliomas were then used for in vivo confirmation. Based on
clinical data, we found that the absence of PRDX1 is
associated with changes in the expression of MET/HGF
signaling components. PRDX1 forms a heterodimer with p38α
mitogen-activated protein kinase 14 (MAPK14), stabilizing
phospho-p38α in glioma cells. This process amplifies
hepatocyte growth factor (HGF)-mediated signaling and
stimulates actin cytoskeleton dynamics that promote glioma
cell migration. Whole-brain high-resolution ultramicroscopy
confirms these findings, indicating that PRDX1 promotes
glioma brain invasion in vivo. Finally, reduced expression
of PRDX1 increased survival in mouse glioma models. Thus,
our preclinical findings suggest that PRDX1 expression
levels may serve as a molecular marker for patients who
could benefit from targeted inhibition of MET/HGF
signaling.},
cin = {V077 / G370 / L101 / F140 / G380 / D170 / C060 / B210 /
C010 / L401 / B060},
ddc = {610},
cid = {I:(DE-He78)V077-20160331 / I:(DE-He78)G370-20160331 /
I:(DE-He78)L101-20160331 / I:(DE-He78)F140-20160331 /
I:(DE-He78)G380-20160331 / I:(DE-He78)D170-20160331 /
I:(DE-He78)C060-20160331 / I:(DE-He78)B210-20160331 /
I:(DE-He78)C010-20160331 / I:(DE-He78)L401-20160331 /
I:(DE-He78)B060-20160331},
pnm = {319H - Addenda (POF3-319H)},
pid = {G:(DE-HGF)POF3-319H},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29582423},
doi = {10.1002/ijc.31404},
url = {https://inrepo02.dkfz.de/record/136833},
}